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Quantitative proteomic analysis of cell envelope preparations under iron starvation stress in Aeromonas hydrophila

Overview of attention for article published in BMC Microbiology, July 2016
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Title
Quantitative proteomic analysis of cell envelope preparations under iron starvation stress in Aeromonas hydrophila
Published in
BMC Microbiology, July 2016
DOI 10.1186/s12866-016-0769-5
Pubmed ID
Authors

Zujie Yao, Zhihong Wang, Lina Sun, Wanxin Li, Yan Shi, Ling Lin, Wenxiong Lin, Xiangmin Lin

Abstract

Iron homeostasis is an essential process over the entire lives of both hosts and bacterial pathogens, and also plays roles in many other metabolic functions. Currently, knowledge is limited on the iron scavenging mechanism of the cell envelope in the aquatic pathogen, Aeromonas hydrophila. To understand the iron homeostasis mechanism in A. hydrophila, a dimethyl labelling based quantitative proteomics method was used to compare the differential expression of cell envelope proteins under iron starvation. A total of 542 cell envelope proteins were identified by LC-MS/MS, with 66 down-regulated and 104 up-regulated proteins. Bioinformatics analysis showed that outer membrane siderophores, heme and iron receptors, periplasmic iron binding proteins, inner membrane ABC transporters and H(+)-ATP synthase subunits increased in abundance while iron-cluster proteins, electron transport chain and redox proteins were down-regulated. Further q-PCR validation, in vivo addition of exogenous metabolites, and an enzyme inhibition assay revealed that redox, the energy generation process, and ATP synthase elevated the susceptibility of A. hydrophila to iron starvation. Our study demonstrates that the redox and energy generation process, and ATP synthase in A. hydrophila may play critical roles in iron acquisition under conditions of iron-stress. An understanding of the iron scavenging mechanism may be helpful for the development of strategies for preventing and treating A. hydrophila infection.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 23%
Student > Doctoral Student 5 23%
Student > Bachelor 2 9%
Other 2 9%
Researcher 2 9%
Other 4 18%
Unknown 2 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 32%
Biochemistry, Genetics and Molecular Biology 6 27%
Computer Science 2 9%
Immunology and Microbiology 1 5%
Psychology 1 5%
Other 2 9%
Unknown 3 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2016.
All research outputs
#7,011,362
of 8,098,931 outputs
Outputs from BMC Microbiology
#1,104
of 1,360 outputs
Outputs of similar age
#217,903
of 257,742 outputs
Outputs of similar age from BMC Microbiology
#80
of 97 outputs
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We're also able to compare this research output to 97 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.