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Proteomic analysis of Chromobacterium violaceum and its adaptability to stress

Overview of attention for article published in BMC Microbiology, December 2015
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Title
Proteomic analysis of Chromobacterium violaceum and its adaptability to stress
Published in
BMC Microbiology, December 2015
DOI 10.1186/s12866-015-0606-2
Pubmed ID
Authors

Diogo Castro, Isabelle Bezerra Cordeiro, Paula Taquita, Marcos Nogueira Eberlin, Jerusa Simone Garcia, Gustavo Henrique M. F. Souza, Marco Aurélio Zezzi Arruda, Edmar V. Andrade, Spartaco A. Filho, J. Lee Crainey, Luis Lopez Lozano, Paulo A. Nogueira, Patrícia P. Orlandi

Abstract

Chromobacterium violaceum (C. violaceum) occurs abundantly in a variety of ecosystems, including ecosystems that place the bacterium under stress. This study assessed the adaptability of C. violaceum by submitting it to nutritional and pH stresses and then analyzing protein expression using bi-dimensional electrophoresis (2-DE) and Maldi mass spectrometry. Chromobacterium violaceum grew best in pH neutral, nutrient-rich medium (reference conditions); however, the total protein mass recovered from stressed bacteria cultures was always higher than the total protein mass recovered from our reference culture. The diversity of proteins expressed (repressed by the number of identifiable 2-DE spots) was seen to be highest in the reference cultures, suggesting that stress reduces the overall range of proteins expressed by C. violaceum. Database comparisons allowed 43 of the 55 spots subjected to Maldi mass spectrometry to be characterized as containing a single identifiable protein. Stress-related expression changes were noted for C. violaceum proteins related to the previously characterized bacterial proteins: DnaK, GroEL-2, Rhs, EF-Tu, EF-P; MCP, homogentisate 1,2-dioxygenase, Arginine deiminase and the ATP synthase β-subunit protein as well as for the ribosomal protein subunits L1, L3, L5 and L6. The ability of C. violaceum to adapt its cellular mechanics to sub-optimal growth and protein production conditions was well illustrated by its regulation of ribosomal protein subunits. With the exception of the ribosomal subunit L3, which plays a role in protein folding and maybe therefore be more useful in stressful conditions, all the other ribosomal subunit proteins were seen to have reduced expression in stressed cultures. Curiously, C. violeaceum cultures were also observed to lose their violet color under stress, which suggests that the violacein pigment biosynthetic pathway is affected by stress. Analysis of the proteomic signatures of stressed C. violaceum indicates that nutrient-starvation and pH stress can cause changes in the expression of the C. violaceum receptors, transporters, and proteins involved with biosynthetic pathways, molecule recycling, energy production. Our findings complement the recent publication of the C. violeaceum genome sequence and could help with the future commercial exploitation of C. violeaceum.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 48 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 11 22%
Student > Ph. D. Student 10 20%
Student > Master 6 12%
Student > Doctoral Student 5 10%
Student > Postgraduate 4 8%
Other 5 10%
Unknown 8 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 27%
Biochemistry, Genetics and Molecular Biology 12 24%
Environmental Science 3 6%
Immunology and Microbiology 3 6%
Chemistry 2 4%
Other 7 14%
Unknown 9 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 July 2016.
All research outputs
#20,336,685
of 22,881,964 outputs
Outputs from BMC Microbiology
#2,694
of 3,195 outputs
Outputs of similar age
#324,964
of 387,673 outputs
Outputs of similar age from BMC Microbiology
#42
of 55 outputs
Altmetric has tracked 22,881,964 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,195 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.