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Temperature-induced variation in gene expression burst size in metazoan cells

Overview of attention for article published in BMC Molecular Biology, November 2015
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Title
Temperature-induced variation in gene expression burst size in metazoan cells
Published in
BMC Molecular Biology, November 2015
DOI 10.1186/s12867-015-0048-2
Pubmed ID
Authors

Ophélie Arnaud, Sam Meyer, Elodie Vallin, Guillaume Beslon, Olivier Gandrillon

Abstract

Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanisms of the underlying microscopic molecular system. By using different clones of chicken erythroid progenitor cells harboring different integration sites of a CMV-driven mCherry protein, we investigated the dynamical behavior of such distributions. We show that, on short term, clone distributions can be quickly regenerated from small population samples with a high accuracy. On longer term, on the contrary, we show variations manifested by correlated fluctuation in the Mean Fluorescence Intensity. In search for a possible cause of this correlation, we demonstrate that in response to small temperature variations cells are able to adjust their gene expression rate: a modest (2 °C) increase in external temperature induces a significant down regulation of mean expression values, with a reverse effect observed when the temperature is decreased. Using a two-state model of gene expression we further demonstrate that temperature acts by modifying the size of transcription bursts, while the burst frequency of the investigated promoter is less systematically affected. For the first time, we report that transcription burst size is a key parameter for gene expression that metazoan cells from homeotherm animals can modify in response to an external thermal stimulus.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 1 5%
Unknown 18 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 37%
Student > Bachelor 3 16%
Student > Doctoral Student 2 11%
Researcher 2 11%
Other 1 5%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 32%
Computer Science 3 16%
Environmental Science 1 5%
Chemical Engineering 1 5%
Veterinary Science and Veterinary Medicine 1 5%
Other 4 21%
Unknown 3 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 August 2016.
All research outputs
#7,063,073
of 8,160,820 outputs
Outputs from BMC Molecular Biology
#143
of 176 outputs
Outputs of similar age
#217,663
of 257,661 outputs
Outputs of similar age from BMC Molecular Biology
#4
of 6 outputs
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So far Altmetric has tracked 176 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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