↓ Skip to main content

An ovarian bioreactor for in vitro culture of the whole bovine ovary: a preliminary report

Overview of attention for article published in Journal of Ovarian Research, August 2016
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 X users

Citations

dimensions_citation
8 Dimensions

Readers on

mendeley
24 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
An ovarian bioreactor for in vitro culture of the whole bovine ovary: a preliminary report
Published in
Journal of Ovarian Research, August 2016
DOI 10.1186/s13048-016-0249-4
Pubmed ID
Authors

Matthew R. Zanotelli, Joseph D. Henningsen, Patrick M. Hopkins, Aaron P. Dederich, Tessa Herman, Tracy J. Puccinelli, Sana M. Salih

Abstract

Improved cancer therapeutics and enhanced cancer survivorship have emphasized the severe long-term side effects of chemotherapy. Specifically, studies have linked many chemotherapy agents with primary ovarian insufficiency, although an exact insult model has not yet been determined. To investigate and ultimately solve this problem, a novel device for extended study of mammalian ovaries in vitro was developed. A bioreactor was fabricated for bovine ovarian culture that provides intravascular delivery of media to the ovary through isolation and cannulation of a main ovarian artery branch. Whole ovaries were cultured in vitro using three methods: (1) continuously supplied fresh culture media, (2) recirculated culture media, or (3) continuously supplied fresh culture media supplemented with 500 nM doxorubicin for 24 or 48 h. TUNEL assay was used to assess apoptotic cell percentages in the three groups as compared to uncultured baseline ovaries. The ovary culture method was shown to maintain cell viability by effectively delivering nutrient-enriched pH-balanced media at a constant flow rate. Lower apoptosis observed in ovaries cultured in continuously supplied fresh culture media illustrates that this culture device and method are the first to sustain whole bovine ovary viability for 48 h. Meanwhile, the increase in the percentage of cell apoptosis with doxorubicin treatment indicates that the device can provide an alternative model for testing chemotherapy and chemoprotection treatments to prevent primary ovarian insufficiency in cancer patients. An ovarian bioreactor with consistent culture media flow through an ovarian vasculature-assisted approach maintains short-term whole bovine ovary viability.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 29%
Student > Master 4 17%
Researcher 4 17%
Student > Bachelor 2 8%
Student > Doctoral Student 2 8%
Other 2 8%
Unknown 3 13%
Readers by discipline Count As %
Engineering 5 21%
Medicine and Dentistry 5 21%
Biochemistry, Genetics and Molecular Biology 4 17%
Agricultural and Biological Sciences 2 8%
Veterinary Science and Veterinary Medicine 2 8%
Other 3 13%
Unknown 3 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 June 2018.
All research outputs
#17,811,816
of 22,881,964 outputs
Outputs from Journal of Ovarian Research
#283
of 591 outputs
Outputs of similar age
#269,411
of 367,308 outputs
Outputs of similar age from Journal of Ovarian Research
#5
of 11 outputs
Altmetric has tracked 22,881,964 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 591 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,308 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.