Title |
A Hypomethylated population of Brassica rapa for forward and reverse Epi-genetics
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Published in |
BMC Plant Biology, October 2012
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DOI | 10.1186/1471-2229-12-193 |
Pubmed ID | |
Authors |
Stephen Amoah, Smita Kurup, Carlos Marcelino Rodriguez Lopez, Sue J Welham, Stephen J Powers, Clare J Hopkins, Michael J Wilkinson, Graham J King |
Abstract |
Epigenetic marks superimposed on the DNA sequence of eukaryote chromosomes provide agility and plasticity in terms of modulating gene expression, ontology, and response to the environment. Modulating the methylation status of cytosine can generate epialleles, which have been detected and characterised at specific loci in several plant systems, and have the potential to generate novel and relatively stable phenotypes. There have been no systematic attempts to explore and utilise epiallelic variation, and so extend the range of phenotypes available for selection in crop improvement. We developed an approach for generating novel epialleles by perturbation of the DNA methylation status. 5- Azacytidine (5-AzaC) provides selective targeting of 5 mCG, which in plants is associated with exonic DNA. Targeted chemical intervention using 5-AzaC has advantages over transgenic or mutant modulation of methyltransferases, allowing stochastic generation of epialleles across the genome. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Sweden | 1 | <1% |
Australia | 1 | <1% |
Unknown | 102 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 27 | 26% |
Student > Ph. D. Student | 22 | 21% |
Other | 8 | 8% |
Professor > Associate Professor | 8 | 8% |
Student > Master | 8 | 8% |
Other | 15 | 14% |
Unknown | 16 | 15% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 63 | 61% |
Biochemistry, Genetics and Molecular Biology | 15 | 14% |
Medicine and Dentistry | 2 | 2% |
Mathematics | 1 | <1% |
Environmental Science | 1 | <1% |
Other | 4 | 4% |
Unknown | 18 | 17% |