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Zinc-induced cardiomyocyte relaxation in a rat model of hyperglycemia is independent of myosin isoform

Overview of attention for article published in Cardiovascular Diabetology, November 2012
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Title
Zinc-induced cardiomyocyte relaxation in a rat model of hyperglycemia is independent of myosin isoform
Published in
Cardiovascular Diabetology, November 2012
DOI 10.1186/1475-2840-11-135
Pubmed ID
Authors

Ting Yi, Yaser Cheema, Sarah M Tremble, Stephen P Bell, Zengyi Chen, Meenakumari Subramanian, Martin M LeWinter, Peter VanBuren, Bradley M Palmer

Abstract

It has been reported previously that diabetic cardiomyopathy can be inhibited or reverted with chronic zinc supplementation. In the current study, we hypothesized that total cardiac calcium and zinc content is altered in early onset diabetes mellitus characterized in part as hyperglycemia (HG) and that exposure of zinc ion (Zn2+) to isolated cardiomyocytes would enhance contraction-relaxation function in HG more so than in nonHG controls. To better control for differential cardiac myosin isoform expression as occurs in rodents after β-islet cell necrosis, hypothyroidism was induced in 16 rats resulting in 100% β-myosin heavy chain expression in the heart. β-Islet cell necrosis was induced in half of the rats by streptozocin administration. After 6 wks of HG, both HG and nonHG controls rats demonstrated similar myofilament performance measured as thin filament calcium sensitivity, native thin filament velocity in the myosin motility assay and contractile velocity and power. Extracellular Zn2+ reduced cardiomyocyte contractile function in both groups, but enhanced relaxation function significantly in the HG group compared to controls. Most notably, a reduction in diastolic sarcomere length with increasing pacing frequencies, i.e., incomplete relaxation, was more pronounced in the HG compared to controls, but was normalized with extracellular Zn2+ application. This is a novel finding implicating that the detrimental effect of HG on cardiomyocyte Ca2+ regulation can be amelioration by Zn2+. Among the many post-translational modifications examined, only phosphorylation of ryanodine receptor (RyR) at S-2808 was significantly higher in HG compared to nonHG. We did not find in our hypothyroid rats any differentiating effects of HG on myofibrillar protein phosphorylation, lysine acetylation, O-linked N-acetylglucosamine and advanced glycated end-products, which are often implicated as complicating factors in cardiac performance due to HG. Our results suggest that the relaxing effects of Zn2+ on cardiomyocyte function are more pronounced in the HG state due an insulin-dependent effect of enhancing removal of cytosolic Ca2+ via SERCA2a or NCX or by reducing Ca2+ influx via L-type channel or Ca2+ leak through the RyR. Investigations into the effects of Zn2+ on these mechanisms are now underway.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 6%
Russia 1 6%
Unknown 16 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 22%
Student > Bachelor 3 17%
Student > Doctoral Student 3 17%
Student > Ph. D. Student 3 17%
Student > Master 2 11%
Other 1 6%
Unknown 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 22%
Agricultural and Biological Sciences 3 17%
Medicine and Dentistry 3 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 11%
Sports and Recreations 1 6%
Other 4 22%
Unknown 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2012.
All research outputs
#7,527,502
of 12,483,518 outputs
Outputs from Cardiovascular Diabetology
#370
of 737 outputs
Outputs of similar age
#74,629
of 142,687 outputs
Outputs of similar age from Cardiovascular Diabetology
#3
of 7 outputs
Altmetric has tracked 12,483,518 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 737 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,687 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.