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The pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa

Overview of attention for article published in Microbial Cell Factories, July 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (74th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

blogs
1 blog

Citations

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45 Dimensions

Readers on

mendeley
59 Mendeley
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Title
The pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa
Published in
Microbial Cell Factories, July 2016
DOI 10.1186/s12934-016-0519-2
Pubmed ID
Authors

Jiao Xue, Lan Wang, Lin Zhang, Srinivasan Balamurugan, Da-Wei Li, Hao Zeng, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li

Abstract

The fast growing photosynthetic microalgae have been widely used in aquaculture, food, health, and biofuels. Recent findings in the diatom has proposed a pivotal role of NADP-malic enzyme in generation of NADPH as an important supply of reducing power for fatty acid biosynthesis. To test the lipogenic malic enzyme for fatty acid synthesis in green algae, here the malic enzyme gene PtME from the oleaginous diatom Phaeodactylum tricornutum was expressed in a representative green microalga Chlorella pyrenoidosa. The engineered C. pyrenoidosa strain showed higher enzymatic activity of malic enzyme which subsequently promoted fatty acid synthesis. The neutral lipid content was significantly increased by up to 3.2-fold than wild type determined by Nile red staining, and total lipid content reached 40.9 % (dry cell weight). The engineered strain exhibited further lipid accumulation subjected to nitrogen deprivation condition. Upon nitrogen deprivation, engineered microalgae accumulated total lipid up to 58.7 % (dry cell weight), a 4.6-fold increase over the wild type cells under normal culture condition. At cellular level, increased volume and number of oil bodies were observed in the engineered microalgal cells. These findings suggested that malic enzyme is a pivotal regulator in lipid accumulation in green microalga C. pyrenoidosa, and presenting a breakthrough of generating ideal algal strains for algal nutrition and biofuels.

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 2%
Unknown 58 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 17%
Student > Master 8 14%
Student > Doctoral Student 7 12%
Researcher 7 12%
Student > Bachelor 6 10%
Other 12 20%
Unknown 9 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 31%
Biochemistry, Genetics and Molecular Biology 17 29%
Environmental Science 3 5%
Immunology and Microbiology 3 5%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Other 2 3%
Unknown 14 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 August 2016.
All research outputs
#1,567,644
of 8,200,733 outputs
Outputs from Microbial Cell Factories
#71
of 574 outputs
Outputs of similar age
#64,168
of 257,425 outputs
Outputs of similar age from Microbial Cell Factories
#2
of 29 outputs
Altmetric has tracked 8,200,733 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 574 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 257,425 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.