Title |
Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing
|
---|---|
Published in |
Virology Journal, November 2012
|
DOI | 10.1186/1743-422x-9-261 |
Pubmed ID | |
Authors |
Kelvin Li, Susmita Shrivastava, Anushka Brownley, Dan Katzel, Jayati Bera, Anh Thu Nguyen, Vishal Thovarai, Rebecca Halpin, Timothy B Stockwell |
Abstract |
In a high-throughput environment, to PCR amplify and sequence a large set of viral isolates from populations that are potentially heterogeneous and continuously evolving, the use of degenerate PCR primers is an important strategy. Degenerate primers allow for the PCR amplification of a wider range of viral isolates with only one set of pre-mixed primers, thus increasing amplification success rates and minimizing the necessity for genome finishing activities. To successfully select a large set of degenerate PCR primers necessary to tile across an entire viral genome and maximize their success, this process is best performed computationally. |
X Demographics
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Country | Count | As % |
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Peru | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
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Portugal | 1 | 1% |
Unknown | 75 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 15 | 20% |
Student > Ph. D. Student | 10 | 13% |
Student > Master | 9 | 12% |
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Professor | 4 | 5% |
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Unknown | 15 | 20% |
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Unspecified | 3 | 4% |
Other | 7 | 9% |
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