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Zymomonas diversity and potential for biofuel production

Overview of attention for article published in Biotechnology for Biofuels, May 2021
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Title
Zymomonas diversity and potential for biofuel production
Published in
Biotechnology for Biofuels, May 2021
DOI 10.1186/s13068-021-01958-2
Pubmed ID
Authors

Magdalena M. Felczak, Robert M. Bowers, Tanja Woyke, Michaela A. TerAvest

Abstract

Zymomonas mobilis is an aerotolerant α-proteobacterium, which has been genetically engineered for industrial purposes for decades. However, a comprehensive comparison of existing strains on the genomic level in conjunction with phenotype analysis has yet to be carried out. We here performed whole-genome comparison of 17 strains including nine that were sequenced in this study. We then compared 15 available Zymomonas strains for their natural abilities to perform under conditions relevant to biofuel synthesis. We tested their growth in anaerobic rich media, as well as growth, ethanol production and xylose utilization in lignocellulosic hydrolysate. We additionally compared their tolerance to isobutanol, flocculation characteristics, and ability to uptake foreign DNA by electroporation and conjugation. Using clustering based on 99% average nucleotide identity (ANI), we classified 12 strains into four clusters based on sequence similarity, while five strains did not cluster with any other strain. Strains belonging to the same 99% ANI cluster showed similar performance while significant variation was observed between the clusters. Overall, conjugation and electroporation efficiencies were poor across all strains, which was consistent with our finding of coding potential for several DNA defense mechanisms, such as CRISPR and restriction-modification systems, across all genomes. We found that strain ATCC31821 (ZM4) had a more diverse plasmid profile than other strains, possibly leading to the unique phenotypes observed for this strain. ZM4 also showed the highest growth of any strain in both laboratory media and lignocellulosic hydrolysate and was among the top 3 strains for isobutanol tolerance and electroporation and conjugation efficiency. Our findings suggest that strain ZM4 has a unique combination of genetic and phenotypic traits that are beneficial for biofuel production and propose investing future efforts in further engineering of ZM4 for industrial purposes rather than exploring new Zymomonas isolates.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 27%
Researcher 5 23%
Lecturer 1 5%
Student > Ph. D. Student 1 5%
Professor 1 5%
Other 2 9%
Unknown 6 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 18%
Agricultural and Biological Sciences 4 18%
Chemical Engineering 2 9%
Social Sciences 1 5%
Neuroscience 1 5%
Other 2 9%
Unknown 8 36%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2021.
All research outputs
#18,689,862
of 21,033,636 outputs
Outputs from Biotechnology for Biofuels
#1,179
of 1,377 outputs
Outputs of similar age
#279,347
of 339,078 outputs
Outputs of similar age from Biotechnology for Biofuels
#1
of 1 outputs
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So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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