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Comparative transcriptome profiling of resistant and susceptible rice genotypes in response to the seedborne pathogen Fusarium fujikuroi

Overview of attention for article published in BMC Genomics, August 2016
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Title
Comparative transcriptome profiling of resistant and susceptible rice genotypes in response to the seedborne pathogen Fusarium fujikuroi
Published in
BMC Genomics, August 2016
DOI 10.1186/s12864-016-2925-6
Pubmed ID
Authors

Slavica Matić, Paolo Bagnaresi, Chiara Biselli, Luigi Orru’, Greice Amaral Carneiro, Ilenia Siciliano, Giampiero Valé, Maria Lodovica Gullino, Davide Spadaro

Abstract

Fusarium fujikuroi is the causal agent of bakanae, the most significant seed-borne disease of rice. Molecular mechanisms regulating defence responses of rice towards this fungus are not yet fully known. To identify transcriptional mechanisms underpinning rice resistance, a RNA-seq comparative transcriptome profiling was conducted on infected seedlings of selected rice genotypes at one and three weeks post germination (wpg). Twelve rice genotypes were screened against bakanae disease leading to the identification of Selenio and Dorella as the most resistant and susceptible cultivars, respectively. Transcriptional changes were more appreciable at 3 wpg, suggesting that this infection stage is essential to study the resistance mechanisms: 3,119 DEGs were found in Selenio and 5,095 in Dorella. PR1, germin-like proteins, glycoside hydrolases, MAP kinases, and WRKY transcriptional factors were up-regulated in the resistant genotype upon infection with F. fujikuroi. Up-regulation of chitinases and down-regulation of MAP kinases and WRKY transcriptional factors were observed in the susceptible genotype. Gene ontology (GO) enrichment analyses detected in Selenio GO terms specific to response to F. fujikuroi: 'response to chitin', 'jasmonic acid biosynthetic process', and 'plant-type hypersensitive response', while Dorella activated different mechanisms, such as 'response to salicylic acid stimulus' and 'gibberellin metabolic process', which was in agreement with the production of gibberellin A3 in Dorella plants. RNA-seq profiling was performed for the first time to analyse response of rice to F. fujikuroi infection. Our findings allowed the identification of genes activated in one- and three- week-old rice seedlings of two genotypes infected with F. fujikuroi. Furthermore, we found the pathways involved in bakanae resistance, such as response to chitin, JA-dependent signalling and hypersensitive response. Collectively, this provides important information to elucidate the molecular and cellular processes occurring in rice during F. fujikuroi infection and to develop bakanae resistant rice germplasm.

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Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 1%
Italy 1 1%
Unknown 86 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 19%
Student > Master 17 19%
Student > Ph. D. Student 16 18%
Student > Bachelor 6 7%
Professor > Associate Professor 4 5%
Other 9 10%
Unknown 19 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 45%
Biochemistry, Genetics and Molecular Biology 15 17%
Nursing and Health Professions 1 1%
Veterinary Science and Veterinary Medicine 1 1%
Energy 1 1%
Other 3 3%
Unknown 27 31%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2016.
All research outputs
#7,121,757
of 8,229,276 outputs
Outputs from BMC Genomics
#5,158
of 5,817 outputs
Outputs of similar age
#214,119
of 252,985 outputs
Outputs of similar age from BMC Genomics
#234
of 264 outputs
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