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Genome wide SNP discovery in flax through next generation sequencing of reduced representation libraries

Overview of attention for article published in BMC Genomics, December 2012
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Citations

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Title
Genome wide SNP discovery in flax through next generation sequencing of reduced representation libraries
Published in
BMC Genomics, December 2012
DOI 10.1186/1471-2164-13-684
Pubmed ID
Authors

Santosh Kumar, Frank M You, Sylvie Cloutier

Abstract

Flax (Linum usitatissimum L.) is a significant fibre and oilseed crop. Current flax molecular markers, including isozymes, RAPDs, AFLPs and SSRs are of limited use in the construction of high density linkage maps and for association mapping applications due to factors such as low reproducibility, intense labour requirements and/or limited numbers. We report here on the use of a reduced representation library strategy combined with next generation Illumina sequencing for rapid and large scale discovery of SNPs in eight flax genotypes. SNP discovery was performed through in silico analysis of the sequencing data against the whole genome shotgun sequence assembly of flax genotype CDC Bethune. Genotyping-by-sequencing of an F6-derived recombinant inbred line population provided validation of the SNPs.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 1%
Netherlands 1 1%
Italy 1 1%
Sweden 1 1%
United States 1 1%
Unknown 80 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 28%
Researcher 23 27%
Student > Master 11 13%
Student > Doctoral Student 5 6%
Student > Bachelor 5 6%
Other 13 15%
Unknown 4 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 58 68%
Biochemistry, Genetics and Molecular Biology 8 9%
Engineering 4 5%
Medicine and Dentistry 3 4%
Computer Science 2 2%
Other 5 6%
Unknown 5 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2012.
All research outputs
#8,581,752
of 11,181,823 outputs
Outputs from BMC Genomics
#4,953
of 6,767 outputs
Outputs of similar age
#203,600
of 307,939 outputs
Outputs of similar age from BMC Genomics
#145
of 213 outputs
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