Title |
Genome wide SNP discovery in flax through next generation sequencing of reduced representation libraries
|
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Published in |
BMC Genomics, December 2012
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DOI | 10.1186/1471-2164-13-684 |
Pubmed ID | |
Authors |
Santosh Kumar, Frank M You, Sylvie Cloutier |
Abstract |
Flax (Linum usitatissimum L.) is a significant fibre and oilseed crop. Current flax molecular markers, including isozymes, RAPDs, AFLPs and SSRs are of limited use in the construction of high density linkage maps and for association mapping applications due to factors such as low reproducibility, intense labour requirements and/or limited numbers. We report here on the use of a reduced representation library strategy combined with next generation Illumina sequencing for rapid and large scale discovery of SNPs in eight flax genotypes. SNP discovery was performed through in silico analysis of the sequencing data against the whole genome shotgun sequence assembly of flax genotype CDC Bethune. Genotyping-by-sequencing of an F6-derived recombinant inbred line population provided validation of the SNPs. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 1% |
Netherlands | 1 | 1% |
Italy | 1 | 1% |
Sweden | 1 | 1% |
United States | 1 | 1% |
Unknown | 89 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 24 | 26% |
Researcher | 23 | 24% |
Student > Master | 13 | 14% |
Student > Doctoral Student | 6 | 6% |
Student > Bachelor | 5 | 5% |
Other | 14 | 15% |
Unknown | 9 | 10% |
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Biochemistry, Genetics and Molecular Biology | 8 | 9% |
Engineering | 4 | 4% |
Medicine and Dentistry | 3 | 3% |
Computer Science | 3 | 3% |
Other | 8 | 9% |
Unknown | 11 | 12% |