Title |
Molecular probe technology detects bacteria without culture
|
---|---|
Published in |
BMC Microbiology, March 2012
|
DOI | 10.1186/1471-2180-12-29 |
Pubmed ID | |
Authors |
Richard W Hyman, Robert P St Onge, Hyunsung Kim, John S Tamaresis, Molly Miranda, Ana Maria Aparicio, Marilyn Fukushima, Nader Pourmand, Linda C Giudice, Ronald W Davis |
Abstract |
Our ultimate goal is to detect the entire human microbiome, in health and in disease, in a single reaction tube, and employing only commercially available reagents. To that end, we adapted molecular inversion probes to detect bacteria using solely a massively multiplex molecular technology. This molecular probe technology does not require growth of the bacteria in culture. Rather, the molecular probe technology requires only a sequence of forty sequential bases unique to the genome of the bacterium of interest. In this communication, we report the first results of employing our molecular probes to detect bacteria in clinical samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 5% |
Mexico | 1 | 2% |
Sweden | 1 | 2% |
Germany | 1 | 2% |
Unknown | 51 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 28 | 49% |
Student > Ph. D. Student | 9 | 16% |
Student > Bachelor | 4 | 7% |
Professor | 4 | 7% |
Professor > Associate Professor | 2 | 4% |
Other | 5 | 9% |
Unknown | 5 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 32 | 56% |
Biochemistry, Genetics and Molecular Biology | 9 | 16% |
Medicine and Dentistry | 4 | 7% |
Chemistry | 3 | 5% |
Immunology and Microbiology | 2 | 4% |
Other | 2 | 4% |
Unknown | 5 | 9% |