Title |
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays
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Published in |
BMC Genomics, August 2008
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DOI | 10.1186/1471-2164-9-390 |
Pubmed ID | |
Authors |
Alexandra Graf, Brigitte Gasser, Martin Dragosits, Michael Sauer, Germán G Leparc, Thomas Tüchler, David P Kreil, Diethard Mattanovich |
Abstract |
DNA Microarrays are regarded as a valuable tool for basic and applied research in microbiology. However, for many industrially important microorganisms the lack of commercially available microarrays still hampers physiological research. Exemplarily, our understanding of protein folding and secretion in the yeast Pichia pastoris is presently widely dependent on conclusions drawn from analogies to Saccharomyces cerevisiae. To close this gap for a yeast species employed for its high capacity to produce heterologous proteins, we developed full genome DNA microarrays for P. pastoris and analyzed the unfolded protein response (UPR) in this yeast species, as compared to S. cerevisiae. |
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