Title |
Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing
|
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Published in |
BMC Genomics, December 2012
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DOI | 10.1186/1471-2164-13-738 |
Pubmed ID | |
Authors |
Shuli Liang, Bin Wang, Li Pan, Yanrui Ye, Minghui He, Shuangyan Han, Suiping Zheng, Xiaoning Wang, Ying Lin |
Abstract |
The methylotrophic yeast Pichia pastoris is widely used as a bioengineering platform for producing industrial and biopharmaceutical proteins, studying protein expression and secretion mechanisms, and analyzing metabolite synthesis and peroxisome biogenesis. With the development of DNA microarray and mRNA sequence technology, the P. pastoris transcriptome has become a research hotspot due to its powerful capability to identify the transcript structures and gain insights into the transcriptional regulation model of cells under protein production conditions. The study of the P. pastoris transcriptome helps to annotate the P. pastoris transcript structures and provide useful information for further improvement of the production of recombinant proteins. |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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India | 1 | <1% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 26 | 15% |
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Student > Doctoral Student | 7 | 4% |
Other | 26 | 15% |
Unknown | 23 | 14% |
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Chemistry | 3 | 2% |
Other | 9 | 5% |
Unknown | 28 | 17% |