Title |
Stool substitute transplant therapy for the eradication of Clostridium difficile infection: ‘RePOOPulating’ the gut
|
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Published in |
Microbiome, January 2013
|
DOI | 10.1186/2049-2618-1-3 |
Pubmed ID | |
Authors |
Elaine O Petrof, Gregory B Gloor, Stephen J Vanner, Scott J Weese, David Carter, Michelle C Daigneault, Eric M Brown, Kathleen Schroeter, Emma Allen-Vercoe |
Abstract |
Fecal bacteriotherapy ('stool transplant') can be effective in treating recurrent Clostridium difficile infection, but concerns of donor infection transmission and patient acceptance limit its use. Here we describe the use of a stool substitute preparation, made from purified intestinal bacterial cultures derived from a single healthy donor, to treat recurrent C. difficile infection that had failed repeated standard antibiotics. Thirty-three isolates were recovered from a healthy donor stool sample. Two patients who had failed at least three courses of metronidazole or vancomycin underwent colonoscopy and the mixture was infused throughout the right and mid colon. Pre-treatment and post-treatment stool samples were analyzed by 16 S rRNA gene sequencing using the Ion Torrent platform. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 37 | 27% |
United Kingdom | 17 | 13% |
Canada | 16 | 12% |
Ireland | 3 | 2% |
Australia | 3 | 2% |
France | 2 | 1% |
Sweden | 2 | 1% |
South Africa | 1 | <1% |
Italy | 1 | <1% |
Other | 10 | 7% |
Unknown | 44 | 32% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 88 | 65% |
Scientists | 30 | 22% |
Practitioners (doctors, other healthcare professionals) | 12 | 9% |
Science communicators (journalists, bloggers, editors) | 6 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 17 | 2% |
Canada | 7 | <1% |
United Kingdom | 7 | <1% |
Denmark | 3 | <1% |
Spain | 2 | <1% |
Sweden | 2 | <1% |
Chile | 1 | <1% |
Brazil | 1 | <1% |
Malaysia | 1 | <1% |
Other | 11 | 1% |
Unknown | 881 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 164 | 18% |
Student > Ph. D. Student | 143 | 15% |
Student > Bachelor | 134 | 14% |
Student > Master | 100 | 11% |
Other | 58 | 6% |
Other | 185 | 20% |
Unknown | 149 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 264 | 28% |
Medicine and Dentistry | 204 | 22% |
Biochemistry, Genetics and Molecular Biology | 109 | 12% |
Immunology and Microbiology | 71 | 8% |
Engineering | 22 | 2% |
Other | 91 | 10% |
Unknown | 172 | 18% |