Title |
Exposure of RML scrapie agent to a sodium percarbonate-based product and sodium dodecyl sulfate renders PrPScprotease sensitive but does not eliminate infectivity
|
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Published in |
BMC Veterinary Research, January 2013
|
DOI | 10.1186/1746-6148-9-8 |
Pubmed ID | |
Authors |
Jodi D Smith, Eric M Nicholson, Gregory H Foster, Justin J Greenlee |
Abstract |
Prions, the causative agents of the transmissible spongiform encephalopathies, are notoriously difficult to inactivate. Current decontamination recommendations by the World Health Organization include prolonged exposure to 1 N sodium hydroxide or > 20,000 ppm sodium hypochlorite, or autoclaving. For decontamination of large stainless steel surfaces and equipment as in abattoirs, for example, these methods are harsh or unsuitable. The current study was designed to evaluate the effectiveness of a commercial product containing sodium percarbonate to inactivate prions. Samples of mouse brain infected with a mouse-adapted strain of the scrapie agent (RML) were exposed to a sodium percarbonate-based product (SPC-P). Treated samples were evaluated for abnormal prion protein (PrPSc)-immunoreactivity by western blot analysis, and residual infectivity by mouse bioassay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 40% |
Student > Bachelor | 3 | 15% |
Professor | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Student > Ph. D. Student | 1 | 5% |
Other | 3 | 15% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 25% |
Biochemistry, Genetics and Molecular Biology | 3 | 15% |
Veterinary Science and Veterinary Medicine | 2 | 10% |
Nursing and Health Professions | 2 | 10% |
Environmental Science | 1 | 5% |
Other | 5 | 25% |
Unknown | 2 | 10% |