Title |
Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology
|
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Published in |
BMC Microbiology, January 2013
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DOI | 10.1186/1471-2180-13-9 |
Pubmed ID | |
Authors |
Greg A Somerville, Richard A Proctor |
Abstract |
Bacterial cultivation requires consideration of three things: The bacterial strain, cultivation medium, and cultivation conditions. Most microbiologists dutifully report their choice of strains and cultivation media in manuscripts; however, these same microbiologists often overlook reporting cultivation conditions. Without this information, it is difficult to determine if cultures were grown aerobically, microaerobically, or anaerobically. To cultivate bacteria aerobically, it is necessary to understand that oxygen does not readily diffuse into culture media; it needs help to get in. Microbiologists can do this by altering the flask-to-medium ratio, rpm of agitation, and/or the concentration of atmospheric oxygen, or by using baffled flasks. |
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Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 2 | 1% |
Germany | 1 | <1% |
Belgium | 1 | <1% |
Canada | 1 | <1% |
Unknown | 162 | 97% |
Demographic breakdown
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Researcher | 22 | 13% |
Student > Master | 20 | 12% |
Student > Bachelor | 17 | 10% |
Professor > Associate Professor | 9 | 5% |
Other | 28 | 17% |
Unknown | 32 | 19% |
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Immunology and Microbiology | 10 | 6% |
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Medicine and Dentistry | 7 | 4% |
Other | 28 | 17% |
Unknown | 35 | 21% |