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Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology

Overview of attention for article published in BMC Microbiology, January 2013
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Title
Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology
Published in
BMC Microbiology, January 2013
DOI 10.1186/1471-2180-13-9
Pubmed ID
Authors

Greg A Somerville, Richard A Proctor

Abstract

Bacterial cultivation requires consideration of three things: The bacterial strain, cultivation medium, and cultivation conditions. Most microbiologists dutifully report their choice of strains and cultivation media in manuscripts; however, these same microbiologists often overlook reporting cultivation conditions. Without this information, it is difficult to determine if cultures were grown aerobically, microaerobically, or anaerobically. To cultivate bacteria aerobically, it is necessary to understand that oxygen does not readily diffuse into culture media; it needs help to get in. Microbiologists can do this by altering the flask-to-medium ratio, rpm of agitation, and/or the concentration of atmospheric oxygen, or by using baffled flasks.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 167 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Germany 1 <1%
Belgium 1 <1%
Canada 1 <1%
Unknown 162 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 39 23%
Researcher 22 13%
Student > Master 20 12%
Student > Bachelor 17 10%
Professor > Associate Professor 9 5%
Other 28 17%
Unknown 32 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 44 26%
Biochemistry, Genetics and Molecular Biology 34 20%
Immunology and Microbiology 10 6%
Engineering 9 5%
Medicine and Dentistry 7 4%
Other 28 17%
Unknown 35 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2013.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from BMC Microbiology
#2,468
of 3,489 outputs
Outputs of similar age
#231,759
of 292,951 outputs
Outputs of similar age from BMC Microbiology
#32
of 43 outputs
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So far Altmetric has tracked 3,489 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
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We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.