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High throughput techniques to reveal the molecular physiology and evolution of digestion in spiders

Overview of attention for article published in BMC Genomics, September 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

news
1 news outlet
twitter
4 tweeters

Citations

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14 Dimensions

Readers on

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21 Mendeley
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Title
High throughput techniques to reveal the molecular physiology and evolution of digestion in spiders
Published in
BMC Genomics, September 2016
DOI 10.1186/s12864-016-3048-9
Pubmed ID
Authors

Felipe J. Fuzita, Martijn W. H. Pinkse, José S. L. Patane, Peter D. E. M. Verhaert, Adriana R. Lopes

Abstract

Spiders are known for their predatory efficiency and for their high capacity of digesting relatively large prey. They do this by combining both extracorporeal and intracellular digestion. Whereas many high throughput ("-omics") techniques focus on biomolecules in spider venom, so far this approach has not yet been applied to investigate the protein composition of spider midgut diverticula (MD) and digestive fluid (DF). We here report on our investigations of both MD and DF of the spider Nephilingis (Nephilengys) cruentata through the use of next generation sequencing and shotgun proteomics. This shows that the DF is composed of a variety of hydrolases including peptidases, carbohydrases, lipases and nuclease, as well as of toxins and regulatory proteins. We detect 25 astacins in the DF. Phylogenetic analysis of the corresponding transcript(s) in Arachnida suggests that astacins have acquired an unprecedented role for extracorporeal digestion in Araneae, with different orthologs used by each family. The results of a comparative study of spiders in distinct physiological conditions allow us to propose some digestion mechanisms in this interesting animal taxon. All the high throughput data allowed the demonstration that DF is a secretion originating from the MD. We identified enzymes involved in the extracellular and intracellular phases of digestion. Besides that, data analyses show a large gene duplication event in Araneae digestive process evolution, mainly of astacin genes. We were also able to identify proteins expressed and translated in the digestive system, which until now had been exclusively associated to venom glands.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 24%
Other 2 10%
Student > Ph. D. Student 2 10%
Researcher 2 10%
Professor 1 5%
Other 3 14%
Unknown 6 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 43%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Biochemistry, Genetics and Molecular Biology 1 5%
Neuroscience 1 5%
Engineering 1 5%
Other 0 0%
Unknown 8 38%

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2017.
All research outputs
#1,002,802
of 12,114,099 outputs
Outputs from BMC Genomics
#463
of 7,131 outputs
Outputs of similar age
#34,771
of 261,135 outputs
Outputs of similar age from BMC Genomics
#11
of 291 outputs
Altmetric has tracked 12,114,099 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,131 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 261,135 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 291 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.