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Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation

Overview of attention for article published in BMC Biology, January 2013
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Title
Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation
Published in
BMC Biology, January 2013
DOI 10.1186/1741-7007-11-4
Pubmed ID
Authors

Tyson A Clark, Xingyu Lu, Khai Luong, Qing Dai, Matthew Boitano, Stephen W Turner, Chuan He, Jonas Korlach

Abstract

DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is 5-methylcytosine, whereas prokaryotes can have 6-methyladenine, 4-methylcytosine, or 5-methylcytosine. Single-molecule, real-time sequencing is capable of directly detecting all three types of modified bases. However, the kinetic signature of 5-methylcytosine is subtle, which presents a challenge for detection. We investigated whether conversion of 5-methylcytosine to 5-carboxylcytosine using the enzyme Tet1 would enhance the kinetic signature, thereby improving detection.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 115 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 3%
United Kingdom 2 2%
Japan 2 2%
Canada 1 <1%
China 1 <1%
Unknown 105 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 21%
Researcher 24 21%
Student > Master 11 10%
Student > Bachelor 10 9%
Professor > Associate Professor 7 6%
Other 17 15%
Unknown 22 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 48 42%
Biochemistry, Genetics and Molecular Biology 28 24%
Medicine and Dentistry 6 5%
Chemistry 5 4%
Immunology and Microbiology 1 <1%
Other 3 3%
Unknown 24 21%