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X Demographics
Mendeley readers
Attention Score in Context
| Title |
High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division
|
|---|---|
| Published in |
BMC Molecular and Cell Biology, September 2016
|
| DOI | 10.1186/s12860-016-0111-y |
| Pubmed ID | |
| Authors |
David Dickerson, Marek Gierliński, Vijender Singh, Etsushi Kitamura, Graeme Ball, Tomoyuki U. Tanaka, Tom Owen-Hughes |
| Abstract |
Genomes of eukaryotes exist as chromatin, and it is known that different chromatin states can influence gene regulation. Chromatin is not a static structure, but is known to be dynamic and vary between cells. In order to monitor the organisation of chromatin in live cells we have engineered fluorescent fusion proteins which recognize specific operator sequences to tag pairs of syntenic gene loci. The separation of these loci was then tracked in three dimensions over time using fluorescence microscopy. We established a work flow for measuring the distance between two fluorescently tagged, syntenic gene loci with a mean measurement error of 63 nm. In general, physical separation was observed to increase with increasing genomic separations. However, the extent to which chromatin is compressed varies for different genomic regions. No correlation was observed between compaction and the distribution of chromatin markers from genomic datasets or with contacts identified using capture based approaches. Variation in spatial separation was also observed within cells over time and between cells. Differences in the conformation of individual loci can persist for minutes in individual cells. Separation of reporter loci was found to be similar in related and unrelated daughter cell pairs. The directly observed physical separation of reporter loci in live cells is highly dynamic both over time and from cell to cell. However, consistent differences in separation are observed over some chromosomal regions that do not correlate with factors known to influence chromatin states. We conclude that as yet unidentified parameters influence chromatin configuration. We also find that while heterogeneity in chromatin states can be maintained for minutes between cells, it is not inherited through cell division. This may contribute to cell-to-cell transcriptional heterogeneity. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 50% |
| Germany | 1 | 10% |
| France | 1 | 10% |
| Unknown | 3 | 30% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 50% |
| Members of the public | 4 | 40% |
| Practitioners (doctors, other healthcare professionals) | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 78 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 78 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 27 | 35% |
| Student > Ph. D. Student | 15 | 19% |
| Student > Bachelor | 10 | 13% |
| Researcher | 9 | 12% |
| Student > Master | 5 | 6% |
| Other | 6 | 8% |
| Unknown | 6 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 40 | 51% |
| Agricultural and Biological Sciences | 21 | 27% |
| Physics and Astronomy | 5 | 6% |
| Computer Science | 1 | 1% |
| Economics, Econometrics and Finance | 1 | 1% |
| Other | 4 | 5% |
| Unknown | 6 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2016.
All research outputs
#7,148,094
of 25,371,288 outputs
Outputs from BMC Molecular and Cell Biology
#234
of 1,233 outputs
Outputs of similar age
#103,543
of 342,729 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#4
of 13 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,729 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.