Title |
Feasibility of using 454 pyrosequencing for studying quasispecies of the whole dengue viral genome
|
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Published in |
BMC Genomics, December 2012
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DOI | 10.1186/1471-2164-13-s7-s7 |
Pubmed ID | |
Authors |
Kwanrutai Chin-inmanu, Aroonroong Suttitheptumrong, Duangjai Sangsrakru, Sithichoke Tangphatsornruang, Somvong Tragoonrung, Prida Malasit, Sumalee Tungpradabkul, Prapat Suriyaphol |
Abstract |
Dengue is the world's most common mosquito-borne viral disease. Poor proofreading by RNA polymerase during its replication results in the accumulation of mutations in its genome. This leads to a diversity of genotypes in the viral population termed quasispecies. Quasispecies play an important role in disease severity. The study of quasispecies in dengue has been hindered because of the requirement for large amounts of cloning and sequencing, which could be overcome by 454 pyrosequencing. In this study, we attempted to demonstrate the feasibility of using 454 pyrosequencing to study genome diversity of dengue virus quasispecies by sequencing a pool of known dengue viral strains. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 33% |
United States | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 4% |
Colombia | 1 | 2% |
France | 1 | 2% |
French Polynesia | 1 | 2% |
Unknown | 45 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 26% |
Student > Ph. D. Student | 13 | 26% |
Student > Bachelor | 8 | 16% |
Student > Master | 5 | 10% |
Lecturer | 3 | 6% |
Other | 6 | 12% |
Unknown | 2 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 48% |
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Medicine and Dentistry | 7 | 14% |
Immunology and Microbiology | 6 | 12% |
Social Sciences | 1 | 2% |
Other | 1 | 2% |
Unknown | 3 | 6% |