Title |
The mutated tegument protein UL7 attenuates the virulence of herpes simplex virus 1 by reducing the modulation of α-4 gene transcription
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Published in |
Virology Journal, September 2016
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DOI | 10.1186/s12985-016-0600-9 |
Pubmed ID | |
Authors |
Xingli Xu, Shengtao Fan, Jienan Zhou, Ying Zhang, Yanchun Che, Hongzhi Cai, Lichun Wang, Lei Guo, Longding Liu, Qihan Li |
Abstract |
UL7, a tegument protein of Herpes Simplex Virus type I (HSV-1), is highly conserved in viral infection and proliferation and has an unknown mechanism of action. A HSV-1 UL7 mutant (UL7-MU) was constructed using the CRISPR-cas9 system. The replication rate and plaque morphology were used to analyze the biological characteristics of the wild-type (WT), UL7-MU and MU-complemented P1 viruses. The virulence of the viruses was evaluated in mice. Real-time RT-qPCR and ChIP assays were used to determine the expression levels of relevant genes. The replication capacity of a recombinant virus (UL7-MU strain) was 10-fold lower than that of the WT strain. The neurovirulence and pathologic effect of the UL7-MU strain were attenuated in infected mice compared with the WT strain. In the latency model, the expression of latency-associated transcript (LAT) in the central nervous system (CNS) and trigeminal nerve was lower in UL7-MU-infected mice than in WT strain-infected mice. The transcription level of the immediate-early gene α-4 in UL7-MU-infected cells was reduced by approximately 2-fold compared with the clear transcriptional peak identified in WT strain-infected Vero cells within 7 h post-infection (p.i.). By modulating the transcription of the α-4 gene, UL7 may be involved in transcriptional regulation through its interaction with the transcript complex structure of the viral genome during HSV-1 infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 10 | 29% |
Researcher | 8 | 24% |
Student > Ph. D. Student | 6 | 18% |
Student > Master | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Other | 2 | 6% |
Unknown | 3 | 9% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 5 | 15% |
Immunology and Microbiology | 5 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Medicine and Dentistry | 2 | 6% |
Other | 5 | 15% |
Unknown | 4 | 12% |