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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction
|
|---|---|
| Published in |
Genome Biology, September 2016
|
| DOI | 10.1186/s13059-016-1045-6 |
| Pubmed ID | |
| Authors |
Alex S Genshaft, Shuqiang Li, Caroline J. Gallant, Spyros Darmanis, Sanjay M. Prakadan, Carly G. K. Ziegler, Martin Lundberg, Simon Fredriksson, Joyce Hong, Aviv Regev, Kenneth J. Livak, Ulf Landegren, Alex K. Shalek |
| Abstract |
We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidigm C1™ system, we profile the transcriptomic and proteomic response of a human breast adenocarcinoma cell line to a chemical perturbation, benchmarking against in situ hybridizations and immunofluorescence staining, as well as recombinant proteins, ERCC Spike-Ins, and population lysate dilutions. Through supervised and unsupervised analyses, we demonstrate synergies enabled by simultaneous measurement of single-cell protein and RNA abundances. Collectively, our generalizable approach highlights the potential for molecular metadata to inform highly-multiplexed single-cell analyses. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 13% |
| Australia | 1 | 13% |
| Germany | 1 | 13% |
| United States | 1 | 13% |
| United Kingdom | 1 | 13% |
| Unknown | 3 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 6 | 75% |
| Members of the public | 1 | 13% |
| Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 291 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 1% |
| Canada | 2 | <1% |
| Denmark | 2 | <1% |
| Sweden | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 281 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 77 | 26% |
| Researcher | 49 | 17% |
| Student > Bachelor | 29 | 10% |
| Student > Master | 19 | 7% |
| Other | 19 | 7% |
| Other | 53 | 18% |
| Unknown | 45 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 85 | 29% |
| Agricultural and Biological Sciences | 56 | 19% |
| Engineering | 20 | 7% |
| Medicine and Dentistry | 15 | 5% |
| Chemistry | 13 | 4% |
| Other | 47 | 16% |
| Unknown | 55 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 February 2024.
All research outputs
#1,497,808
of 25,374,917 outputs
Outputs from Genome Biology
#1,201
of 4,467 outputs
Outputs of similar age
#26,321
of 328,307 outputs
Outputs of similar age from Genome Biology
#23
of 56 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,307 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.