Title |
Ovine reference materials and assays for prion genetic testing
|
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Published in |
BMC Veterinary Research, April 2010
|
DOI | 10.1186/1746-6148-6-23 |
Pubmed ID | |
Authors |
Michael P Heaton, Kreg A Leymaster, Theodore S Kalbfleisch, Brad A Freking, Timothy PL Smith, Michael L Clawson, William W Laegreid |
Abstract |
Genetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests for scoring PRNP codons are essential tools for eradicating scrapie and for evaluating rare alleles for increased resistance to disease. In addition to those associated with scrapie, there are dozens more PRNP polymorphisms that may occur in various flocks. If not accounted for, these sites may cause base-pair mismatching with oligonucleotides used in DNA testing. Thus, the fidelity of scrapie genetic testing is enhanced by knowing the position and frequency of PRNP polymorphisms in targeted flocks. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 31% |
Student > Ph. D. Student | 4 | 15% |
Student > Doctoral Student | 2 | 8% |
Student > Bachelor | 2 | 8% |
Other | 2 | 8% |
Other | 3 | 12% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 42% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Medicine and Dentistry | 2 | 8% |
Immunology and Microbiology | 1 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 2 | 8% |
Unknown | 5 | 19% |