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Isolation and genomic characterization of SfI, a serotype-converting bacteriophage of Shigella flexneri

Overview of attention for article published in BMC Microbiology, February 2013
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Title
Isolation and genomic characterization of SfI, a serotype-converting bacteriophage of Shigella flexneri
Published in
BMC Microbiology, February 2013
DOI 10.1186/1471-2180-13-39
Pubmed ID
Authors

Qiangzheng Sun, Ruiting Lan, Yiting Wang, Jianping Wang, Yan Wang, Peijing Li, Pengcheng Du, Jianguo Xu

Abstract

ABSTRACT: BACKGROUND: All Shigella flexneri serotypes except serotype 6 share a common O-antigen tetrasaccharide backbone and nearly all variation between serotypes are due to glucosyl and/or O-acetyl modifications of the common O units mediated by glycosyltransferases encoded by serotype-converting bacteriophages. Several S. flexneri serotype-converting phages including SfV, SfX, Sf6 and SfII have been isolated and characterized. However, S. flexneri serotype-converting phage SfI which encodes a type I modification of serotype 1 (1a, 1b, 1c and 1d) had not yet been characterized. RESULTS: The SfI phage was induced and purified from a S. flexneri serotype 1a clinical strain 019. Electron microscopy showed that the SfI phage has a hexagonal head and a long contractile tail, characteristic of the members of Myoviridae family. SfI can convert serotype Y to serotype 1a and serotype X to serotype 1d, but cannot convert 10 other S. flexneri serotypes (1a, 1b, 2a, 2b, 3a, 3b, 4a, 4b, 5a, Xv) tested, suggesting that SfI has a narrow host range. Similar to other S. flexneri serotype-converting phages, SfI integrates into the tRNA-thrW gene adjacent to proA of the host chromosome when lysogenized. The complete sequence of the SfI genome was 38,389 bp, encoding 66 open reading frames and two tRNA genes. Phage SfI shares significant homology with S. flexneri phage SfV, Escherichia coli prophage e14 and lambda, and is classified into the lambdoid phage family. SfI was found to use a cos mechanism for DNA packaging similar to that of phage SfV. CONCLUSIONS: SfI contains features of lambdoid phages and is closely related to S. flexneri phage SfV, E. coli prophage e14 and lambda. The characterization of SfI enhances our understanding of serotype conversion of S. flexneri.

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Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 7%
Unknown 27 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 38%
Student > Ph. D. Student 5 17%
Student > Master 3 10%
Student > Bachelor 2 7%
Student > Doctoral Student 1 3%
Other 2 7%
Unknown 5 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 31%
Biochemistry, Genetics and Molecular Biology 7 24%
Immunology and Microbiology 3 10%
Environmental Science 1 3%
Business, Management and Accounting 1 3%
Other 3 10%
Unknown 5 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2013.
All research outputs
#9,905,538
of 12,372,633 outputs
Outputs from BMC Microbiology
#1,254
of 1,804 outputs
Outputs of similar age
#98,791
of 141,545 outputs
Outputs of similar age from BMC Microbiology
#5
of 5 outputs
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