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Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase

Overview of attention for article published in Biotechnology for Biofuels, April 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

blogs
1 blog
patent
1 patent

Citations

dimensions_citation
21 Dimensions

Readers on

mendeley
32 Mendeley
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Title
Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
Published in
Biotechnology for Biofuels, April 2016
DOI 10.1186/s13068-016-0496-z
Pubmed ID
Authors

Lei Zhang, Yajing Liang, Wei Wu, Xiaoming Tan, Xuefeng Lu

Abstract

Propane, a major component of liquid petroleum gas (LPG) derived from fossil fuels, has widespread applications in vehicles, cooking, and ambient heating. Given the concerns about fossil fuel depletion and carbon emission, exploiting alternative and renewable source of propane have become attractive. In this study, we report the construction of a novel propane biosynthetic pathway in Escherichia coli. We constructed an aldehyde reductases (ALR)-deprived E. coli strain BW25113(DE3) Δ13 via genetic engineering, which produced sufficient isobutyraldehyde precursors and finally achieved de novo synthesis of propane (91 μg/L) by assembling the engineered valine pathway and cyanobacterial aldehyde-deformylating oxygenase (ADO). Additionally, after extensive screening of ADO mutants generated by engineering the active center to accommodate branched-chain isobutyraldehyde, we identified two ADO mutants (I127G, I127G/A48G) which exhibited higher catalytic activity for isobutyraldehyde and improved propane productivity by three times (267 μg/L). The propane biosynthetic pathway constructed here through the engineered valine pathway can produce abundant isobutyraldehyde for ADO and overcome the low availability of precursors in propane production. Furthermore, the rational design aiming at the ADO active center illustrates the plasticity and catalytic potential of ADO. These results together highlight the potential for developing a microbial biomanufacturing platform for propane.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 3%
Sweden 1 3%
China 1 3%
Unknown 29 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 25%
Researcher 8 25%
Student > Master 5 16%
Student > Bachelor 4 13%
Other 1 3%
Other 2 6%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 34%
Biochemistry, Genetics and Molecular Biology 8 25%
Engineering 2 6%
Medicine and Dentistry 2 6%
Chemical Engineering 1 3%
Other 2 6%
Unknown 6 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2019.
All research outputs
#2,562,966
of 16,073,243 outputs
Outputs from Biotechnology for Biofuels
#181
of 1,185 outputs
Outputs of similar age
#55,343
of 272,229 outputs
Outputs of similar age from Biotechnology for Biofuels
#2
of 13 outputs
Altmetric has tracked 16,073,243 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,185 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,229 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.