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Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management

Overview of attention for article published in BMC Genomics, October 2016
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Title
Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management
Published in
BMC Genomics, October 2016
DOI 10.1186/s12864-016-2759-2
Pubmed ID
Authors

M. Constanza Mannino, Máximo Rivarola, Alejandra C. Scannapieco, Sergio González, Marisa Farber, Jorge L. Cladera, Silvia B. Lanzavecchia

Abstract

Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid species, a deeper analysis on D. longicaudata's genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions. We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized. The information obtained here will significantly contribute to the development of D. longicaudata functional genomics, genetics and population-based genome studies. Thousands of new microsatellite markers were identified as toolkits for population genetics analysis. The transcriptome characterized here is the starting point to elucidate the molecular bases of the sex determination mechanism in this species.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 3%
Unknown 31 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 28%
Student > Master 7 22%
Other 3 9%
Student > Ph. D. Student 2 6%
Student > Doctoral Student 2 6%
Other 3 9%
Unknown 6 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 31%
Biochemistry, Genetics and Molecular Biology 7 22%
Medicine and Dentistry 2 6%
Nursing and Health Professions 2 6%
Engineering 2 6%
Other 2 6%
Unknown 7 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2016.
All research outputs
#18,475,157
of 22,893,031 outputs
Outputs from BMC Genomics
#8,198
of 10,670 outputs
Outputs of similar age
#242,031
of 319,855 outputs
Outputs of similar age from BMC Genomics
#172
of 247 outputs
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So far Altmetric has tracked 10,670 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
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We're also able to compare this research output to 247 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.