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A high-density genetic map reveals variation in recombination rate across the genome of Daphnia magna

Overview of attention for article published in BMC Genomic Data, October 2016
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Title
A high-density genetic map reveals variation in recombination rate across the genome of Daphnia magna
Published in
BMC Genomic Data, October 2016
DOI 10.1186/s12863-016-0445-7
Pubmed ID
Authors

Marinela Dukić, Daniel Berner, Marius Roesti, Christoph R. Haag, Dieter Ebert

Abstract

Recombination rate is an essential parameter for many genetic analyses. Recombination rates are highly variable across species, populations, individuals and different genomic regions. Due to the profound influence that recombination can have on intraspecific diversity and interspecific divergence, characterization of recombination rate variation emerges as a key resource for population genomic studies and emphasises the importance of high-density genetic maps as tools for studying genome biology. Here we present such a high-density genetic map for Daphnia magna, and analyse patterns of recombination rate across the genome. A F2 intercross panel was genotyped by Restriction-site Associated DNA sequencing to construct the third-generation linkage map of D. magna. The resulting high-density map included 4037 markers covering 813 scaffolds and contigs that sum up to 77 % of the currently available genome draft sequence (v2.4) and 55 % of the estimated genome size (238 Mb). Total genetic length of the map presented here is 1614.5 cM and the genome-wide recombination rate is estimated to 6.78 cM/Mb. Merging genetic and physical information we consistently found that recombination rate estimates are high towards the peripheral parts of the chromosomes, while chromosome centres, harbouring centromeres in D. magna, show very low recombination rate estimates. Due to its high-density, the third-generation linkage map for D. magna can be coupled with the draft genome assembly, providing an essential tool for genome investigation in this model organism. Thus, our linkage map can be used for the on-going improvements of the genome assembly, but more importantly, it has enabled us to characterize variation in recombination rate across the genome of D. magna for the first time. These new insights can provide a valuable assistance in future studies of the genome evolution, mapping of quantitative traits and population genetic studies.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 27%
Researcher 9 19%
Student > Master 8 17%
Student > Bachelor 7 15%
Student > Doctoral Student 2 4%
Other 2 4%
Unknown 7 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 56%
Biochemistry, Genetics and Molecular Biology 9 19%
Business, Management and Accounting 1 2%
Environmental Science 1 2%
Economics, Econometrics and Finance 1 2%
Other 1 2%
Unknown 8 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2016.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from BMC Genomic Data
#605
of 1,204 outputs
Outputs of similar age
#202,021
of 325,709 outputs
Outputs of similar age from BMC Genomic Data
#9
of 21 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,204 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.