Title |
Pathway-focused bioassays and transcriptome analysis contribute to a better activity monitoring of complex herbal remedies
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Published in |
BMC Genomics, February 2013
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DOI | 10.1186/1471-2164-14-133 |
Pubmed ID | |
Authors |
Angela Klein, Oliver A Wrulich, Marcel Jenny, Peter Gruber, Kathrin Becker, Dietmar Fuchs, Johanna M Gostner, Florian Überall |
Abstract |
Transcriptome analysis in combination with pathway-focused bioassays is suggested to be a helpful approach for gaining deeper insights into the complex mechanisms of action of herbal multicomponent preparations in living cells. The polyherbalism based concept of Tibetan and Ayurvedic medicine considers therapeutic efficacy through multi-target effects. A polyherbal Indo-Tibetan preparation, Padma 28, approved by the Swiss drug authorities (Swissmedic Nr. 58436), was applied to a more detailed dissection of mechanism of action in human hepatoma HepG2 cells. Cell-free and cell-based assays were employed to evaluate the antioxidant capacity. Genome-wide expression profiling was done by applying Human Genome U133 Plus 2.0 Affymetrix arrays. Pathway- and network-oriented analysis elucidated the affected biological processes. The results were validated using reporter gene assays and quantitative real-time PCR. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 28% |
Researcher | 4 | 16% |
Student > Bachelor | 2 | 8% |
Other | 2 | 8% |
Professor > Associate Professor | 2 | 8% |
Other | 5 | 20% |
Unknown | 3 | 12% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Chemistry | 2 | 8% |
Business, Management and Accounting | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Other | 2 | 8% |
Unknown | 5 | 20% |