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Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Overview of attention for article published in Microbial Cell Factories, February 2013
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Title
Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions
Published in
Microbial Cell Factories, February 2013
DOI 10.1186/1475-2859-12-18
Pubmed ID
Authors

Kenji Manabe, Yasushi Kageyama, Takuya Morimoto, Eri Shimizu, Hiroki Takahashi, Shigehiko Kanaya, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara

Abstract

The Bacillus subtilis genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular enzymes under batch fermentation conditions. We predicted that deletion of the gene for RocG, a bi-functional protein that acts as a glutamate dehydrogenase and an indirect repressor of glutamate synthesis, would improve glutamate metabolism, leading to further increased enzyme production. However, deletion of rocG dramatically decreased production of the alkaline cellulase Egl-237 in strain MGB874 (strain 874∆rocG).

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Indonesia 1 2%
Brazil 1 2%
India 1 2%
China 1 2%
Unknown 60 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 16 25%
Student > Ph. D. Student 14 22%
Student > Master 9 14%
Student > Doctoral Student 6 9%
Other 4 6%
Other 9 14%
Unknown 7 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 51%
Biochemistry, Genetics and Molecular Biology 16 25%
Immunology and Microbiology 2 3%
Chemistry 2 3%
Business, Management and Accounting 1 2%
Other 3 5%
Unknown 8 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 March 2013.
All research outputs
#20,185,720
of 22,701,287 outputs
Outputs from Microbial Cell Factories
#1,351
of 1,585 outputs
Outputs of similar age
#169,376
of 192,549 outputs
Outputs of similar age from Microbial Cell Factories
#11
of 11 outputs
Altmetric has tracked 22,701,287 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,585 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 192,549 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.