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Whole genome sequencing uncovers a novel IND-16 metallo-β-lactamase from an extensively drug-resistant Chryseobacterium indologenes strain J31

Overview of attention for article published in Gut Pathogens, October 2016
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Title
Whole genome sequencing uncovers a novel IND-16 metallo-β-lactamase from an extensively drug-resistant Chryseobacterium indologenes strain J31
Published in
Gut Pathogens, October 2016
DOI 10.1186/s13099-016-0130-4
Pubmed ID
Authors

Tingting Wang, Xiawei Jiang, Chunyan Feng, Ang Li, Huihui Dong, Shaoqiang Wu, Beiwen Zheng

Abstract

Chryseobacterium indologenes is an emerging opportunistic pathogen in hospital-acquired infection, which is intrinsically resistant to most antimicrobial agents against gram-negative bacteria. In the purpose of extending our understanding of the resistance mechanism of C. indologenes, we sequenced and analyzed the genome of an extensively antibiotic resistant C. indologenes strain, isolated from a Chinese prostate cancer patient. We also investigated the presence of antibiotic resistance genes, particularly metallo-β-lactamase (MBL) genes, and performed a comparative genomic analysis with other Chryseobacterium species. 16s rRNA sequencing indicated the isolate belongs to C. indologenes. We assembled a total of 1095M bp clean-filtered reads into 171 contigs by de novo assembly. The draft genome of C. indologenes J31 consisted of 5,830,795 bp with a GC content of 36.9 %. RAST analysis revealed the genome contained 5196 coding sequences (CDSs), 28 rRNAs, 81 tRNAs and 114 pseudogenes. We detected 90 antibiotic resistance genes from different drug classes in the whole genome. Notably, a novel blaIND allele blaIND-16 was identified, which shared 99 % identity with blaIND-8 and blaIND-10. By comparing strain J31 genome to the closely four related neighbors in the genus Chryseobacterium, we identified 2634 conserved genes, and 1449 unique genes. In this study, we described the whole genome sequence of C. indologenes strain J31. Numerous resistance determinants were detected in the genome and might be responsible for the extensively antibiotic resistance of this strain. Comparative genomic analysis revealed the presence of considerable strain-specific genes which would contribute to the distinctive characteristics of strain J31. Our study provides the insight of the multidrug resistance mechanism in genus Chryseobacterium.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 36%
Student > Master 2 18%
Student > Bachelor 1 9%
Student > Doctoral Student 1 9%
Other 1 9%
Other 1 9%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 27%
Medicine and Dentistry 2 18%
Immunology and Microbiology 2 18%
Agricultural and Biological Sciences 1 9%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Other 0 0%
Unknown 2 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2016.
All research outputs
#6,486,177
of 8,547,393 outputs
Outputs from Gut Pathogens
#165
of 227 outputs
Outputs of similar age
#173,633
of 249,232 outputs
Outputs of similar age from Gut Pathogens
#9
of 15 outputs
Altmetric has tracked 8,547,393 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 227 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.0. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.