Title |
Comparison of microbial populations in the small intestine, large intestine and feces of healthy horses using terminal restriction fragment length polymorphism
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Published in |
BMC Research Notes, March 2013
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DOI | 10.1186/1756-0500-6-91 |
Pubmed ID | |
Authors |
Angelika Schoster, Luis Guillermo Arroyo, Henry Rolf Staempfli, Jeffrey Scott Weese |
Abstract |
The composition of the microbiota of the equine intestinal tract is complex. Determining whether the microbial composition of fecal samples is representative of proximal compartments of the digestive tract could greatly simplify future studies. The objectives of this study were to compare the microbial populations of the duodenum, ileum, cecum, colon and rectum (feces) within and between healthy horses, and to determine whether rectal (fecal) samples are representative of proximal segments of the gastrointestinal tract. Intestinal samples were collected from ten euthanized horses. 16S rRNA gene PCR-based TRFLP was used to investigate microbiota richness in various segments of the gastrointestinal tract, and dice similarity indices were calculated to compare the samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Peru | 1 | 1% |
Unknown | 84 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 18 | 21% |
Student > Bachelor | 11 | 13% |
Student > Ph. D. Student | 10 | 12% |
Researcher | 7 | 8% |
Student > Doctoral Student | 4 | 5% |
Other | 14 | 16% |
Unknown | 21 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 27 | 32% |
Veterinary Science and Veterinary Medicine | 18 | 21% |
Medicine and Dentistry | 6 | 7% |
Biochemistry, Genetics and Molecular Biology | 5 | 6% |
Immunology and Microbiology | 2 | 2% |
Other | 2 | 2% |
Unknown | 25 | 29% |