Title |
Angiopoietin-2 impairs collateral artery growth associated with the suppression of the infiltration of macrophages in mouse hindlimb ischaemia
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Published in |
Journal of Translational Medicine, October 2016
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DOI | 10.1186/s12967-016-1055-x |
Pubmed ID | |
Authors |
Xiaoyong Tan, Kai Yan, Meiping Ren, Ni Chen, Yongjie Li, Xin Deng, Liqun Wang, Rong Li, Mao Luo, Yong Liu, Yan Liu, Jianbo Wu |
Abstract |
Angiopoietin-2 (Ang-2), a ligand of the Tie-2 receptor, plays an important role in maintaining endothelial cells and in destabilizing blood vessels. Collateral artery growth (arteriogenesis) is a key adaptive response to arterial occlusion. It is unknown whether the destabilization of blood vessels by Ang-2 can affect arteriogenesis and modulate mononuclear cell function. This study aimed to investigate the effects of Ang-2 on collateral artery growth. Hindlimb ischaemia model was produced in C57BL/6 mice by femoral artery ligation. Blood flow perfusion was measured using a laser Doppler perfusion imager quantitative RT-PCR analysis was applied to identify the level of angiogenic factors. After the induction of hindlimb ischaemia, blood flow recovery was impaired in mice treated with recombinant Ang-2 protein; this was accompanied by a reduction of peri-collateral macrophage infiltration. In addition, quantitative RT-PCR analysis revealed that Ang-2 treatment decreased monocyte chemotactic protein-1 (MCP-1), platelet-derived growth factor-BB (PDGF-BB) mRNA levels in ischaemic adductor muscles. Ang-2 can lead to macrophage M1/M2 polarization shift inhibition in the ischaemic muscles. Furthermore, Ang-2 reduced the in vitro inflammatory response in macrophages and vascular cells involved in arteriogenesis. Our results demonstrate that Ang-2 is essential for efficient arteriogenesis, which controls macrophage infiltration. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 18% |
Student > Postgraduate | 3 | 18% |
Student > Doctoral Student | 1 | 6% |
Student > Master | 1 | 6% |
Professor | 1 | 6% |
Other | 2 | 12% |
Unknown | 6 | 35% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 18% |
Biochemistry, Genetics and Molecular Biology | 2 | 12% |
Medicine and Dentistry | 2 | 12% |
Computer Science | 1 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Other | 2 | 12% |
Unknown | 6 | 35% |