Title |
The mutation rate of mycobacterial repetitive unit loci in strains of M. tuberculosisfrom cynomolgus macaque infection
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Published in |
BMC Genomics, March 2013
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DOI | 10.1186/1471-2164-14-145 |
Pubmed ID | |
Authors |
Mark N Ragheb, Christopher B Ford, Michael R Chase, Philana Ling Lin, JoAnne L Flynn, Sarah M Fortune |
Abstract |
Mycobacterial interspersed repetitive units (MIRUs) are minisatellites within the Mycobacterium tuberculosis (Mtb) genome. Copy number variation (CNV) in MIRU loci is used for epidemiological typing, making the rate of variation important for tracking the transmission of Mtb strains. In this study, we developed and assessed a whole-genome sequencing (WGS) approach to detect MIRU CNV in Mtb. We applied this methodology to a panel of Mtb strains isolated from the macaque model of tuberculosis (TB), the animal model that best mimics human disease. From these data, we have estimated the rate of MIRU variation in the host environment, providing a benchmark rate for future epidemiologic work. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United Kingdom | 1 | 2% |
Unknown | 63 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 18 | 28% |
Student > Ph. D. Student | 13 | 20% |
Student > Master | 8 | 13% |
Student > Doctoral Student | 5 | 8% |
Professor | 4 | 6% |
Other | 7 | 11% |
Unknown | 9 | 14% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 13% |
Immunology and Microbiology | 7 | 11% |
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Mathematics | 3 | 5% |
Other | 7 | 11% |
Unknown | 12 | 19% |