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The C-terminal domain of TPX2 is made of alpha-helical tandem repeats

Overview of attention for article published in BMC Molecular and Cell Biology, October 2016
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  • Good Attention Score compared to outputs of the same age and source (75th percentile)

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2 Wikipedia pages
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Title
The C-terminal domain of TPX2 is made of alpha-helical tandem repeats
Published in
BMC Molecular and Cell Biology, October 2016
DOI 10.1186/s12900-016-0070-8
Pubmed ID
Authors

Luis Sanchez-Pulido, Laurent Perez, Steffen Kuhn, Isabelle Vernos, Miguel A. Andrade-Navarro

Abstract

TPX2 (Targeting Protein for Xklp2) is essential for spindle assembly, activation of the mitotic kinase Aurora A and for triggering microtubule nucleation. Homologs of TPX2 in Chordata and plants were previously identified. Currently, proteins of the TPX2 family have little structural information and only small parts are covered by defined protein domains. We have used computational sequence analyses and structural predictions of proteins of the TPX2 family, supported with Circular Dichroism (CD) measurements. Here, we report our finding that the C-terminal domain of TPX2, which is responsible of its microtubule nucleation capacity and is conserved in all members of the family, is actually formed by tandem repeats, covering well above 2/3 of the protein. We propose that this region forms a flexible solenoid involved in protein-protein interactions. Structural prediction and molecular modeling, combined with Circular Dichroism (CD) measurements reveal a predominant alpha-helical content. Furthermore, we identify full length homologs in fungi and shorter homologs with a different domain organization in diptera (including a paralogous expansion in Drosophila). Our results, represent the first computational and biophysical analysis of the TPX2 proteins family and help understand the structure and evolution of this conserved protein family to direct future structural studies.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 19%
Student > Bachelor 6 19%
Student > Doctoral Student 3 9%
Student > Master 3 9%
Librarian 2 6%
Other 2 6%
Unknown 10 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 19%
Psychology 5 16%
Agricultural and Biological Sciences 4 13%
Immunology and Microbiology 2 6%
Computer Science 1 3%
Other 3 9%
Unknown 11 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2023.
All research outputs
#6,754,661
of 25,374,917 outputs
Outputs from BMC Molecular and Cell Biology
#220
of 1,233 outputs
Outputs of similar age
#94,913
of 321,047 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#2
of 8 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 321,047 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.