Title |
Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos
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Published in |
Journal of Animal Science and Biotechnology, March 2013
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DOI | 10.1186/2049-1891-4-12 |
Pubmed ID | |
Authors |
Minhua Hu, Hengxi Wei, Jingfeng Zhang, Yinshan Bai, Fenglei Gao, Li Li, Shouquan Zhang |
Abstract |
Production of chimeric mice is a useful tool for the elucidation of gene function. After successful isolation of embryonic stem (ES) cell lines, there are many methods for producing chimeras, including co-culture with the embryos, microinjection of the ES cells into pre-implantation embryos, and use of tetraploid embryos to generate the full ES-derived transgenic mice. Here, we aimed to generate the transgenic ES cell line, compare the production efficiency of chimeric mice and its proportion to yield the male chimeric mice by microinjected ES cells into 4- to 8-cell and blastocysts embryos with the application of Piezo-Micromanipulator (PMM), and trace the fate of the injected ES cells. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 3% |
Portugal | 1 | 3% |
Switzerland | 1 | 3% |
Unknown | 36 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 9 | 23% |
Student > Ph. D. Student | 7 | 18% |
Researcher | 5 | 13% |
Student > Bachelor | 2 | 5% |
Student > Postgraduate | 2 | 5% |
Other | 3 | 8% |
Unknown | 11 | 28% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 15 | 38% |
Biochemistry, Genetics and Molecular Biology | 8 | 21% |
Medicine and Dentistry | 3 | 8% |
Immunology and Microbiology | 1 | 3% |
Engineering | 1 | 3% |
Other | 0 | 0% |
Unknown | 11 | 28% |