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Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release

Overview of attention for article published in BMC Microbiology, November 2016
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Title
Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release
Published in
BMC Microbiology, November 2016
DOI 10.1186/s12866-016-0881-6
Pubmed ID
Authors

Joseph C. Bryant, Ridge C. Dabbs, Katie L. Oswalt, Lindsey R. Brown, Jason W. Rosch, Keun S. Seo, Janet R. Donaldson, Larry S. McDaniel, Justin A. Thornton

Abstract

Streptococcus pneumoniae is one of the leading causes of community acquired pneumonia and acute otitis media. Certain aspects of S. pneumoniae's virulence are dependent upon expression and release of the protein toxin pneumolysin (PLY) and upon the activity of the peroxide-producing enzyme, pyruvate oxidase (SpxB). We investigated the possible synergy of these two proteins and identified that release of PLY is enhanced by expression of SpxB prior to stationary phase growth. Mutants lacking the spxB gene were defective in PLY release and complementation of spxB restored PLY release. This was demonstrated by cytotoxic effects of sterile filtered supernatants upon epithelial cells and red blood cells. Additionally, peroxide production appeared to contribute to the mechanism of PLY release since a significant correlation was found between peroxide production and PLY release among a panel of clinical isolates. Exogenous addition of H2O2 failed to induce PLY release and catalase supplementation prevented PLY release in some strains, indicating peroxide may exert its effect intracellularly or in a strain-dependent manner. SpxB expression did not trigger bacterial cell death or LytA-dependent autolysis, but did predispose cells to deoxycholate lysis. Here we demonstrate a novel link between spxB expression and PLY release. These findings link liberation of PLY toxin to oxygen availability and pneumococcal metabolism.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 47 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 17%
Student > Master 8 17%
Student > Bachelor 5 11%
Student > Doctoral Student 4 9%
Researcher 3 6%
Other 8 17%
Unknown 11 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 30%
Immunology and Microbiology 11 23%
Agricultural and Biological Sciences 3 6%
Medicine and Dentistry 2 4%
Business, Management and Accounting 1 2%
Other 6 13%
Unknown 10 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2017.
All research outputs
#14,870,535
of 22,901,818 outputs
Outputs from BMC Microbiology
#1,604
of 3,197 outputs
Outputs of similar age
#188,250
of 313,012 outputs
Outputs of similar age from BMC Microbiology
#25
of 60 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,197 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,012 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.