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Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines

Overview of attention for article published in Microbial Cell Factories, August 2011
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)

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1 blog
3 patents


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233 Mendeley
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Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
Published in
Microbial Cell Factories, August 2011
DOI 10.1186/1475-2859-10-s1-s4
Pubmed ID

Luis G Bermúdez-Humarán, Pascale Kharrat, Jean-Marc Chatel, Philippe Langella


Food-grade Lactic Acid Bacteria (LAB) have been safely consumed for centuries by humans in fermented foods. Thus, they are good candidates to develop novel oral vectors, constituting attractive alternatives to attenuated pathogens, for mucosal delivery strategies. Herein, this review summarizes our research, up until now, on the use of LAB as mucosal delivery vectors for therapeutic proteins and DNA vaccines. Most of our work has been based on the model LAB Lactococcus lactis, for which we have developed efficient genetic tools, including expression signals and host strains, for the heterologous expression of therapeutic proteins such as antigens, cytokines and enzymes. Resulting recombinant lactococci strains have been tested successfully for their prophylactic and therapeutic effects in different animal models: i) against human papillomavirus type 16 (HPV-16)-induced tumors in mice, ii) to partially prevent a bovine β-lactoglobulin (BLG)-allergic reaction in mice and iii) to regulate body weight and food consumption in obese mice. Strikingly, all of these tools have been successfully transposed to the Lactobacillus genus, in recent years, within our laboratory. Notably, anti-oxidative Lactobacillus casei strains were constructed and tested in two chemically-induced colitis models. In parallel, we also developed a strategy based on the use of L. lactis to deliver DNA at the mucosal level, and were able to show that L. lactis is able to modulate the host response through DNA delivery. Today, we consider that all of our consistent data, together with those obtained by other groups, demonstrate and reinforce the interest of using LAB, particularly lactococci and lactobacilli strains, to develop novel therapeutic protein mucosal delivery vectors which should be tested now in human clinical trials.

Mendeley readers

The data shown below were compiled from readership statistics for 233 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 <1%
France 2 <1%
Sweden 1 <1%
Netherlands 1 <1%
Kazakhstan 1 <1%
Indonesia 1 <1%
Malaysia 1 <1%
Canada 1 <1%
Mexico 1 <1%
Other 3 1%
Unknown 219 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 48 21%
Student > Ph. D. Student 44 19%
Student > Master 41 18%
Student > Bachelor 28 12%
Student > Doctoral Student 10 4%
Other 34 15%
Unknown 28 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 106 45%
Biochemistry, Genetics and Molecular Biology 36 15%
Immunology and Microbiology 17 7%
Medicine and Dentistry 15 6%
Engineering 5 2%
Other 17 7%
Unknown 37 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2020.
All research outputs
of 18,942,198 outputs
Outputs from Microbial Cell Factories
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Outputs of similar age
of 168,078 outputs
Outputs of similar age from Microbial Cell Factories
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Altmetric has tracked 18,942,198 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,353 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,078 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them