↓ Skip to main content

A quantitative PCR method for measuring absolute telomere length

Overview of attention for article published in Biological Procedures Online, January 2011
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#2 of 135)
  • High Attention Score compared to outputs of the same age (92nd percentile)

Mentioned by

blogs
1 blog
twitter
1 tweeter
patent
4 patents

Citations

dimensions_citation
282 Dimensions

Readers on

mendeley
706 Mendeley
citeulike
1 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
A quantitative PCR method for measuring absolute telomere length
Published in
Biological Procedures Online, January 2011
DOI 10.1186/1480-9222-13-3
Pubmed ID
Authors

Nathan J O'Callaghan, Michael Fenech

Abstract

We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative measurement of telomere length (TL) but modified by introducing an oligomer standard to measure aTL. The method describes the oligomer standards, the generation of the standard curve and the calculations required to calculate aTL from the qPCR data. The necessary controls and performance characteristics of the assay are described in detail and compared relative to other methods for measuring TL. Typical results for this assay for a variety of human tissue samples are provided as well as a troubleshooting schedule. This method allows high throughput measurement of aTL using small amounts of DNA making it amenable for molecular epidemiological studies. Compared to the traditional relative TL qPCR assays, the aTL method described in this protocol enables a more direct comparison of results between experiments within and between laboratories.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 706 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 8 1%
United Kingdom 5 <1%
India 3 <1%
France 2 <1%
Brazil 2 <1%
Japan 2 <1%
New Zealand 1 <1%
Turkey 1 <1%
Belarus 1 <1%
Other 6 <1%
Unknown 675 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 157 22%
Researcher 119 17%
Student > Master 111 16%
Student > Bachelor 77 11%
Student > Doctoral Student 48 7%
Other 112 16%
Unknown 82 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 242 34%
Biochemistry, Genetics and Molecular Biology 142 20%
Medicine and Dentistry 89 13%
Neuroscience 26 4%
Immunology and Microbiology 14 2%
Other 78 11%
Unknown 115 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2020.
All research outputs
#1,295,005
of 17,538,071 outputs
Outputs from Biological Procedures Online
#2
of 135 outputs
Outputs of similar age
#12,276
of 162,386 outputs
Outputs of similar age from Biological Procedures Online
#1
of 1 outputs
Altmetric has tracked 17,538,071 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 135 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 162,386 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them