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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A quantitative PCR method for measuring absolute telomere length
|
|---|---|
| Published in |
Biological Procedures Online, January 2011
|
| DOI | 10.1186/1480-9222-13-3 |
| Pubmed ID | |
| Authors |
Nathan J O'Callaghan, Michael Fenech |
| Abstract |
We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative measurement of telomere length (TL) but modified by introducing an oligomer standard to measure aTL. The method describes the oligomer standards, the generation of the standard curve and the calculations required to calculate aTL from the qPCR data. The necessary controls and performance characteristics of the assay are described in detail and compared relative to other methods for measuring TL. Typical results for this assay for a variety of human tissue samples are provided as well as a troubleshooting schedule. This method allows high throughput measurement of aTL using small amounts of DNA making it amenable for molecular epidemiological studies. Compared to the traditional relative TL qPCR assays, the aTL method described in this protocol enables a more direct comparison of results between experiments within and between laboratories. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 810 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | <1% |
| United Kingdom | 5 | <1% |
| India | 2 | <1% |
| Brazil | 2 | <1% |
| France | 2 | <1% |
| Japan | 2 | <1% |
| Turkey | 1 | <1% |
| New Zealand | 1 | <1% |
| Belarus | 1 | <1% |
| Other | 6 | <1% |
| Unknown | 782 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 165 | 20% |
| Researcher | 138 | 17% |
| Student > Master | 121 | 15% |
| Student > Bachelor | 87 | 11% |
| Student > Doctoral Student | 52 | 6% |
| Other | 122 | 15% |
| Unknown | 125 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 250 | 31% |
| Biochemistry, Genetics and Molecular Biology | 175 | 22% |
| Medicine and Dentistry | 96 | 12% |
| Neuroscience | 26 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 17 | 2% |
| Other | 84 | 10% |
| Unknown | 162 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 June 2022.
All research outputs
#1,646,447
of 22,699,621 outputs
Outputs from Biological Procedures Online
#4
of 165 outputs
Outputs of similar age
#9,457
of 182,537 outputs
Outputs of similar age from Biological Procedures Online
#1
of 2 outputs
Altmetric has tracked 22,699,621 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 165 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 182,537 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them