Title |
Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii
|
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Published in |
BMC Genomics, May 2010
|
DOI | 10.1186/1471-2164-11-329 |
Pubmed ID | |
Authors |
Jochen Schmid, Dirk Müller-Hagen, Thomas Bekel, Laura Funk, Ulf Stahl, Volker Sieber, Vera Meyer |
Abstract |
The plant pathogenic basidiomycete Sclerotium rolfsii produces the industrially exploited exopolysaccharide scleroglucan, a polymer that consists of (1 --> 3)-beta-linked glucose with a (1 --> 6)-beta-glycosyl branch on every third unit. Although the physicochemical properties of scleroglucan are well understood, almost nothing is known about the genetics of scleroglucan biosynthesis. Similarly, the biosynthetic pathway of oxalate, the main by-product during scleroglucan production, has not been elucidated yet. In order to provide a basis for genetic and metabolic engineering approaches, we studied scleroglucan and oxalate biosynthesis in S. rolfsii using different transcriptomic approaches. |
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Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 2% |
Japan | 2 | 2% |
Austria | 1 | 1% |
Germany | 1 | 1% |
United States | 1 | 1% |
Unknown | 76 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 27% |
Student > Ph. D. Student | 14 | 17% |
Professor | 10 | 12% |
Professor > Associate Professor | 6 | 7% |
Student > Master | 6 | 7% |
Other | 16 | 19% |
Unknown | 9 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 45 | 54% |
Biochemistry, Genetics and Molecular Biology | 15 | 18% |
Immunology and Microbiology | 3 | 4% |
Chemistry | 2 | 2% |
Materials Science | 2 | 2% |
Other | 5 | 6% |
Unknown | 11 | 13% |