Title |
Designing deep sequencing experiments: detecting structural variation and estimating transcript abundance
|
---|---|
Published in |
BMC Genomics, June 2010
|
DOI | 10.1186/1471-2164-11-385 |
Pubmed ID | |
Authors |
Ali Bashir, Vikas Bansal, Vineet Bafna |
Abstract |
Massively parallel DNA sequencing technologies have enabled the sequencing of several individual human genomes. These technologies are also being used in novel ways for mRNA expression profiling, genome-wide discovery of transcription-factor binding sites, small RNA discovery, etc. The multitude of sequencing platforms, each with their unique characteristics, pose a number of design challenges, regarding the technology to be used and the depth of sequencing required for a particular sequencing application. Here we describe a number of analytical and empirical results to address design questions for two applications: detection of structural variations from paired-end sequencing and estimating mRNA transcript abundance. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 5 | 3% |
United Kingdom | 4 | 2% |
Belgium | 3 | 2% |
Brazil | 2 | 1% |
Malaysia | 1 | <1% |
Italy | 1 | <1% |
Hong Kong | 1 | <1% |
Australia | 1 | <1% |
Netherlands | 1 | <1% |
Other | 4 | 2% |
Unknown | 174 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 73 | 37% |
Student > Ph. D. Student | 43 | 22% |
Professor > Associate Professor | 21 | 11% |
Student > Master | 13 | 7% |
Other | 11 | 6% |
Other | 24 | 12% |
Unknown | 12 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 136 | 69% |
Biochemistry, Genetics and Molecular Biology | 13 | 7% |
Medicine and Dentistry | 11 | 6% |
Computer Science | 4 | 2% |
Mathematics | 4 | 2% |
Other | 14 | 7% |
Unknown | 15 | 8% |