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Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection

Overview of attention for article published in Genetic Vaccines and Therapy, May 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (71st percentile)

Mentioned by

blogs
1 blog

Citations

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10 Dimensions

Readers on

mendeley
35 Mendeley
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Title
Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection
Published in
Genetic Vaccines and Therapy, May 2009
DOI 10.1186/1479-0556-7-6
Pubmed ID
Authors

Christopher Bradburne, Kelly Robertson, Dzung Thach

Abstract

Nucleofection is an emerging technology for delivery of nucleic acids into both the cytoplasm and nucleus of eukaryotic cells with high efficiency. This makes it an ideal technology for gene delivery and siRNA applications. A 96-well format has recently been made available for high-throughput nucleofection, however conditions must be optimized for delivery into each specific cell type. Screening each 96-well plate can be expensive, and descriptions of methods and outcomes to determine the best conditions are lacking in the literature. Here we employ simple methods, including cell counting, microscopy, viability and cytotoxicity assays to describe the minimal experimental methods required to optimize nucleofection conditions for a given cell line.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 3%
Unknown 34 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 23%
Researcher 8 23%
Student > Bachelor 3 9%
Other 3 9%
Student > Master 3 9%
Other 6 17%
Unknown 4 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 46%
Medicine and Dentistry 4 11%
Biochemistry, Genetics and Molecular Biology 3 9%
Chemistry 3 9%
Immunology and Microbiology 1 3%
Other 3 9%
Unknown 5 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2010.
All research outputs
#5,603,952
of 22,705,019 outputs
Outputs from Genetic Vaccines and Therapy
#13
of 43 outputs
Outputs of similar age
#26,032
of 92,517 outputs
Outputs of similar age from Genetic Vaccines and Therapy
#1
of 3 outputs
Altmetric has tracked 22,705,019 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 43 research outputs from this source. They receive a mean Attention Score of 4.5. This one scored the same or higher as 30 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 92,517 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them