↓ Skip to main content

Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply

Overview of attention for article published in Biotechnology for Biofuels, November 2016
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

Mentioned by

twitter
10 tweeters

Citations

dimensions_citation
48 Dimensions

Readers on

mendeley
159 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply
Published in
Biotechnology for Biofuels, November 2016
DOI 10.1186/s13068-016-0663-2
Pubmed ID
Authors

Frauke Kracke, Bernardino Virdis, Paul V. Bernhardt, Korneel Rabaey, Jens O. Krömer

Abstract

Microbial electrosynthesis is a novel approach that aims at shifting the cellular metabolism towards electron-dense target products by extracellular electron supply. Many organisms including several acetogenic bacteria have been shown to be able to consume electrical current. However, suitable hosts for relevant industrial processes are yet to be discovered, and major knowledge gaps about the underlying fundamental processes still remain. In this paper, we present the first report of electron uptake by the Gram-positive, ethanol-producing acetogen, Clostridium autoethanogenum. Under heterotrophic conditions, extracellular electron supply induced a significant metabolic shift away from acetate. In electrically enhanced fermentations on fructose, acetate production was cut by more than half, while production of lactate and 2,3-butanediol increased by 35-fold and threefold, respectively. The use of mediators with different redox potential revealed a direct dependency of the metabolic effect on the redox potential at which electrons are supplied. Only electrons delivered at a redox potential low enough to reduce ferredoxin caused the reported effect. Production in acetogenic organisms is usually challenged by cellular energy limitations if the target product does not lead to a net energy gain as in the case of acetate. The presented results demonstrate a significant shift of carbon fluxes away from acetate towards the products, lactate and 2,3-butanediol, induced by small electricity input (~0.09 mol of electrons per mol of substrate). This presents a simple and attractive method to optimize acetogenic fermentations for production of chemicals and fuels using electrochemical techniques. The relationship between metabolic shift and redox potential of electron feed gives an indication of possible electron-transfer mechanisms and helps to prioritize further research efforts.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 159 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
New Zealand 1 <1%
France 1 <1%
Australia 1 <1%
Unknown 156 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 39 25%
Researcher 35 22%
Student > Master 20 13%
Student > Bachelor 11 7%
Student > Doctoral Student 8 5%
Other 20 13%
Unknown 26 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 32 20%
Environmental Science 27 17%
Agricultural and Biological Sciences 22 14%
Chemical Engineering 15 9%
Engineering 14 9%
Other 15 9%
Unknown 34 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2017.
All research outputs
#3,424,082
of 15,045,269 outputs
Outputs from Biotechnology for Biofuels
#255
of 1,144 outputs
Outputs of similar age
#93,422
of 383,908 outputs
Outputs of similar age from Biotechnology for Biofuels
#24
of 113 outputs
Altmetric has tracked 15,045,269 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,144 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 383,908 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 113 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.