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Rapid plasmid replicon typing by real time PCR melting curve analysis

Overview of attention for article published in BMC Microbiology, April 2013
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3 X users

Citations

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75 Mendeley
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Title
Rapid plasmid replicon typing by real time PCR melting curve analysis
Published in
BMC Microbiology, April 2013
DOI 10.1186/1471-2180-13-83
Pubmed ID
Authors

Maikel Boot, Susanne Raadsen, Paul HM Savelkoul, Christina Vandenbroucke-Grauls

Abstract

Genes encoding Extended Spectrum Beta Lactamases are usually located on transferable plasmids. Each plasmid contains its own replication mechanism. Carattoli et al. developed an extended PCR-based replicon typing method to characterize and identify the replicons of the major plasmid incompatibility groups in Enterobacteriaceae. Based on this method, we designed a rapid approach with amplicon detection by real-time melting curve analysis. This method appeared to be fast, sensitive, less laborious, less prone to contamination and applicable in a routine laboratory environment.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 3%
Mexico 1 1%
Portugal 1 1%
India 1 1%
Unknown 70 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 20%
Student > Master 13 17%
Researcher 11 15%
Professor 6 8%
Student > Doctoral Student 5 7%
Other 14 19%
Unknown 11 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 21%
Medicine and Dentistry 10 13%
Biochemistry, Genetics and Molecular Biology 9 12%
Immunology and Microbiology 8 11%
Engineering 3 4%
Other 9 12%
Unknown 20 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2013.
All research outputs
#16,297,667
of 25,736,439 outputs
Outputs from BMC Microbiology
#1,600
of 3,514 outputs
Outputs of similar age
#124,409
of 210,535 outputs
Outputs of similar age from BMC Microbiology
#31
of 50 outputs
Altmetric has tracked 25,736,439 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,514 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 210,535 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.