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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus
|
|---|---|
| Published in |
BMC Veterinary Research, November 2016
|
| DOI | 10.1186/s12917-016-0875-5 |
| Pubmed ID | |
| Authors |
Mohamed A. Shalaby, Ayman El-Deeb, Mohamed El-Tholoth, Donata Hoffmann, Claus-Peter Czerny, Frank T. Hufert, Manfred Weidmann, Ahmed Abd El Wahed |
| Abstract |
Lumpy skin disease virus (LSDV) is a Capripoxvirus infecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed. The LSDV RPA assay was performed at 42 °C and detected down to 179 DNA copies/reaction in a maximum of 15 min. Unspecific amplification was observed with neither LSDV-negative samples (n = 12) nor nucleic acid preparations from orf virus, bovine papular stomatitis virus, cowpoxvirus, Peste des petits ruminants and Blue tongue virus (serotypes 1, 6 and 8). The clinical sensitivity of the LSDV RPA assay matched 100 % (n = 22) to real-time PCR results. In addition, the LSDV RPA assay detected sheep and goat poxviruses. The LSDV RPA assay is a rapid and sensitive test that could be implemented in field or at quarantine stations for the identification of LSDV infected case. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Unknown | 87 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 15% |
| Researcher | 13 | 15% |
| Student > Master | 11 | 13% |
| Student > Bachelor | 7 | 8% |
| Professor > Associate Professor | 5 | 6% |
| Other | 14 | 16% |
| Unknown | 25 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 19% |
| Veterinary Science and Veterinary Medicine | 13 | 15% |
| Biochemistry, Genetics and Molecular Biology | 7 | 8% |
| Medicine and Dentistry | 6 | 7% |
| Engineering | 3 | 3% |
| Other | 10 | 11% |
| Unknown | 32 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2016.
All research outputs
#18,483,671
of 22,903,988 outputs
Outputs from BMC Veterinary Research
#1,927
of 3,056 outputs
Outputs of similar age
#235,316
of 311,565 outputs
Outputs of similar age from BMC Veterinary Research
#28
of 48 outputs
Altmetric has tracked 22,903,988 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,056 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,565 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.