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Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning

Overview of attention for article published in Stem Cell Research & Therapy, October 2016
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  • Good Attention Score compared to outputs of the same age and source (65th percentile)

Mentioned by

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2 tweeters
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2 Facebook pages

Citations

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18 Dimensions

Readers on

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34 Mendeley
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Title
Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning
Published in
Stem Cell Research & Therapy, October 2016
DOI 10.1186/s13287-016-0409-x
Pubmed ID
Authors

Kwan-Long Mung, Yat-Ping Tsui, Evelyn Wing-Yin Tai, Ying-Shing Chan, Daisy Kwok-Yan Shum, Graham Ka-Hon Shea

Abstract

Bone marrow stromal cells (BMSCs) are attractive as a source of neural progenitors for ex vivo generation of neurons and glia. Limited numbers of this subpopulation, however, hinder translation into autologous cell-based therapy. Here, we demonstrate rapid and efficient conditioning with hypoxia to enrich for these neural progenitor cells prior to further expansion in neurosphere culture. Adherent cultures of BMSCs (rat/human) were subjected to 1 % oxygen for 24 h and then subcultured as neurospheres with epidermal growth factor (EGF) and basic fibroblast growth factor supplementation. Neurospheres and cell progeny were monitored immunocytochemically for marker expression. To generate Schwann cell-like cells, neurospheres were plated out and exposed to gliogenic medium. The resulting cells were co-cultured with purified dorsal root ganglia (rat) neurons and then tested for commitment to the Schwann cell fate. Fate-committed Schwann cells were subjected to in vitro myelination assay. Transient hypoxic treatment increased the size and number of neurospheres generated from both rat and human BMSCs. This effect was EGF-dependent and attenuated with the EGF receptor inhibitor erlotinib. Hypoxia did not affect the capacity of neurospheres to generate neuron- or glia-like precursors. Human Schwann cell-like cells generated from hypoxia-treated BMSCs demonstrated expression of S100β /p75 and capacity for myelination in vitro. Enhancing the yield of neural progenitor cells with hypoxic preconditioning of BMSCs in vitro but without inherent risks of genetic manipulation provides a platform for upscaling production of neural cell derivatives for clinical application in cell-based therapy.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 21%
Student > Ph. D. Student 5 15%
Student > Doctoral Student 3 9%
Researcher 3 9%
Professor 2 6%
Other 5 15%
Unknown 9 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 21%
Agricultural and Biological Sciences 5 15%
Medicine and Dentistry 5 15%
Neuroscience 2 6%
Psychology 1 3%
Other 2 6%
Unknown 12 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 November 2016.
All research outputs
#4,161,437
of 8,674,965 outputs
Outputs from Stem Cell Research & Therapy
#354
of 753 outputs
Outputs of similar age
#138,697
of 297,056 outputs
Outputs of similar age from Stem Cell Research & Therapy
#10
of 29 outputs
Altmetric has tracked 8,674,965 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 753 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 297,056 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.