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A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination

Overview of attention for article published in BMC Microbiology, April 2013
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

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4 X users
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1 patent

Citations

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27 Dimensions

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84 Mendeley
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Title
A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination
Published in
BMC Microbiology, April 2013
DOI 10.1186/1471-2180-13-86
Pubmed ID
Authors

Masahito Ishikawa, Katsutoshi Hori

Abstract

For the disruption of a target gene in molecular microbiology, unmarked mutagenesis is preferable to marked mutagenesis because the former method raises no concern about the polar effect and leaves no selection marker. In contrast to naturally competent bacteria, there is no useful method for introducing an unmarked mutation into a large gene of non-competent bacteria. Nevertheless, large genes encoding huge proteins exist in diverse bacteria and are interesting and important for physiology and potential applications. Here we present a new method for introducing an unmarked mutation into such large genes of non-competent Gram-negative bacteria.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 1%
United States 1 1%
Germany 1 1%
Unknown 81 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 21 25%
Student > Ph. D. Student 15 18%
Researcher 10 12%
Student > Bachelor 8 10%
Student > Doctoral Student 6 7%
Other 14 17%
Unknown 10 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 37%
Biochemistry, Genetics and Molecular Biology 23 27%
Immunology and Microbiology 6 7%
Medicine and Dentistry 3 4%
Computer Science 3 4%
Other 6 7%
Unknown 12 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2016.
All research outputs
#6,753,656
of 25,371,288 outputs
Outputs from BMC Microbiology
#699
of 3,489 outputs
Outputs of similar age
#53,690
of 209,583 outputs
Outputs of similar age from BMC Microbiology
#9
of 48 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 3,489 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,583 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.