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A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination

Overview of attention for article published in BMC Microbiology, January 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (78th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

twitter
5 tweeters
patent
1 patent

Citations

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25 Dimensions

Readers on

mendeley
79 Mendeley
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Title
A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination
Published in
BMC Microbiology, January 2013
DOI 10.1186/1471-2180-13-86
Pubmed ID
Authors

Masahito Ishikawa, Katsutoshi Hori

Abstract

For the disruption of a target gene in molecular microbiology, unmarked mutagenesis is preferable to marked mutagenesis because the former method raises no concern about the polar effect and leaves no selection marker. In contrast to naturally competent bacteria, there is no useful method for introducing an unmarked mutation into a large gene of non-competent bacteria. Nevertheless, large genes encoding huge proteins exist in diverse bacteria and are interesting and important for physiology and potential applications. Here we present a new method for introducing an unmarked mutation into such large genes of non-competent Gram-negative bacteria.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 1%
United States 1 1%
Germany 1 1%
Unknown 76 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 21 27%
Student > Ph. D. Student 15 19%
Researcher 10 13%
Student > Bachelor 8 10%
Professor > Associate Professor 6 8%
Other 10 13%
Unknown 9 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 39%
Biochemistry, Genetics and Molecular Biology 21 27%
Immunology and Microbiology 6 8%
Medicine and Dentistry 3 4%
Computer Science 3 4%
Other 4 5%
Unknown 11 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2016.
All research outputs
#5,482,416
of 22,601,066 outputs
Outputs from BMC Microbiology
#577
of 3,154 outputs
Outputs of similar age
#62,701
of 297,808 outputs
Outputs of similar age from BMC Microbiology
#34
of 177 outputs
Altmetric has tracked 22,601,066 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,154 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 297,808 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 177 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.