Title |
Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials
|
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Published in |
BMC Microbiology, April 2013
|
DOI | 10.1186/1471-2180-13-88 |
Pubmed ID | |
Authors |
Begoña Bedrina, Sonia Macián, Inmaculada Solís, Roberto Fernández-Lafuente, Eva Baldrich, Guillermo Rodríguez |
Abstract |
Legionellosis is an uncommon form of pneumonia. After a clinical encounter, the necessary antibiotic treatment is available if the diagnosis is made early in the illness. Before the clinical encounter, early detection of the main pathogen involved, Legionella pneumophila, in hazardous environments is important in preventing infectious levels of this bacterium. In this study a qualitative test based on combined magnetic immunocapture and enzyme-immunoassay for the fast detection of Legionella pneumophila in water samples was compared with the standard method, in both comparative and collaborative trials. The test was based on the use of anti-Legionella pneumophila antibodies immobilized on magnetic microspheres. The final protocol included concentration by filtration, resuspension and immunomagnetic capture. The whole assay took less than 1 hour to complete. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 2% |
Italy | 1 | 2% |
Slovenia | 1 | 2% |
Unknown | 59 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 19% |
Student > Master | 11 | 18% |
Researcher | 6 | 10% |
Student > Bachelor | 6 | 10% |
Student > Doctoral Student | 4 | 6% |
Other | 12 | 19% |
Unknown | 11 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 23% |
Biochemistry, Genetics and Molecular Biology | 8 | 13% |
Immunology and Microbiology | 7 | 11% |
Medicine and Dentistry | 6 | 10% |
Engineering | 4 | 6% |
Other | 9 | 15% |
Unknown | 14 | 23% |