RT @GenomeBiology: Extru-seq, from Kwon, Kim, Hwang, Lee & co is a method for predicting off-target Cas9 sites that combines the benefits o…
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"Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches" Kwon J, Kim M, Hwang W [..] Lee JK. Genome Biol 2023-01-10. https://t.co/AWRqiu4XCx #CRISPR https://t.co/AGIyEuDUGG
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RT @GenomeBiology: Extru-seq, from Kwon, Kim, Hwang, Lee & co is a method for predicting off-target Cas9 sites that combines the benefits o…
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Extru-seq, from Kwon, Kim, Hwang, Lee & co is a method for predicting off-target Cas9 sites that combines the benefits of cell-based and in vitro approaches. DNA from lysed cells extruded through a membrane is incubated with Cas9-sgRNA complex & se