↓ Skip to main content

The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

Overview of attention for article published in Nutrition Journal, November 2016
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (78th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

Mentioned by

news
1 news outlet

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
16 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects
Published in
Nutrition Journal, November 2016
DOI 10.1186/s12937-016-0212-7
Pubmed ID
Authors

Sifan Zhai, Mingzuo Zhao, Changcheng Zhou, Fenggang Lu, Huankai Zhang, Li Na, Shanshan Feng, Xiaoxin Qiang, Yong Du

Abstract

To study the association between the expression of H3K27me3 and ACat2 (a folate metabolic protein), in order to elucidate the protective mechanism of folic acid (FA) in neural tube defects (NTDs). Eighteen female SD rats were randomly divided into normal, NTD and FA group. NTD group was induced by all-trans retinoic acid (ATRA) at E10d. FA group was fed with FA supplementation since 2 weeks before pregnancy, followed by ATRA induction. At E15d, FA level in the embryonic neural tube was determined by ELISA. Neural stem cells (NSCs) were isolated. Cell proliferation was compared by CCK-8 assay. The differentiation potency was assessed by immunocytochemical staining. H3K27me3 expression was measured by immunofluorescence method and Western blot. ACat2 mRNA expression was detected by qRT-PCR. Cultured NSCs formed numerous Nestin-positive neurospheres. After 5 days, they differentiated into NSE-positive neurons and GFAP-positive astrocytes. When compared with controls, the FA level in NTD group was significantly lower, the ability of cell proliferation and differentiation was significantly reduced, H3K27me3 expression was increased, and ACat2 mRNA expression was decreased (P <0.05). The intervention of FA notably reversed these changes (P <0.05). H3K27me3 expression was negatively correlated with the FA level (rs = -0.908, P <0.01) and ACat2 level (rs = -0.879, P <0.01) in the neural tube. The increased H3K27me3 expression might cause a disorder of folate metabolic pathway by silencing ACat2 expression, leading to reduced proliferation and differentiation of NSCs, and ultimately the occurrence of NTD. FA supplementation may reverse this process.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 19%
Student > Postgraduate 3 19%
Researcher 3 19%
Student > Master 2 13%
Unspecified 1 6%
Other 1 6%
Unknown 3 19%
Readers by discipline Count As %
Medicine and Dentistry 4 25%
Biochemistry, Genetics and Molecular Biology 3 19%
Agricultural and Biological Sciences 2 13%
Unspecified 1 6%
Nursing and Health Professions 1 6%
Other 2 13%
Unknown 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2016.
All research outputs
#1,308,877
of 8,713,305 outputs
Outputs from Nutrition Journal
#433
of 920 outputs
Outputs of similar age
#61,443
of 298,885 outputs
Outputs of similar age from Nutrition Journal
#6
of 17 outputs
Altmetric has tracked 8,713,305 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 920 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.1. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,885 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.